Clearance of yeast prions by misfolded multi-transmembrane proteins

Chie Arai, Hiroshi Kurahashi, Masao Ishiwata, Keita Oishi, Yoshikazu Nakamura

Research output: Contribution to journalArticle

Abstract

Accumulation of misfolded proteins in the endoplasmic reticulum (ER) induces the stress response to protect cells against toxicity by the unfolded protein response (UPR), heat shock response (HSR), and ER-associated degradation pathways. Here, we found that over-production of C-terminally truncated multi-transmembrane (MTM) mutant proteins triggers HSR, but not UPR, and clearance of yeast prions [PSI+] and [URE3]. One of the mutant MTM proteins, Dip5ΔC-v82, produces a disabled amino-acid permease. Fluorescence microscopy analysis revealed abnormal accumulation of Dip5ΔC-v82 in the ER. Importantly, the mutant defective in the GET pathway, which functions for ER membrane insertion of tail-anchored proteins, failed to translocate Dip5ΔC-v82 to the ER and disabled Dip5ΔC-v82-mediated prion clearance. These findings suggest that the GET pathway plays a pivotal role in quality assurance of MTM proteins, and entraps misfolded MTM proteins into ER compartments, leading to loss-of-prion through a yet undefined mechanism.

Original languageEnglish
Pages (from-to)1223-1232
Number of pages10
JournalBiochimie
Volume95
Issue number6
DOIs
Publication statusPublished - 2013 Jun

Keywords

  • GET complex
  • Heat shock response
  • Misfolded multi-trans membranes
  • Yeast prion

ASJC Scopus subject areas

  • Biochemistry

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    Arai, C., Kurahashi, H., Ishiwata, M., Oishi, K., & Nakamura, Y. (2013). Clearance of yeast prions by misfolded multi-transmembrane proteins. Biochimie, 95(6), 1223-1232. https://doi.org/10.1016/j.biochi.2013.01.009