Classical protein kinase C(s) regulates targeting of synaptotagmin IX to the endocytic recycling compartment

Yael Haberman, Idit Ziv, Yaara Gorzalczany, Mitsunori Fukuda, Ronit Sagi-Eisenberg

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Neuronal and non-neuronal tissues show distinctly different intracellular localization of synaptotagmin (Syt) homologues. Therefore, cell type-specific mechanisms are likely to direct Syt homologues to their final cellular destinations. Syt IX localizes to dense core vesicles in PC12 cells. However, in the rat basophilic leukemia (RBL-2H3) mast cell line, as well as in CHO cells, Syt IX is localized at the endocytic recycling compartment (ERC). We show that targeting of Syt IX to the ERC involves constitutive trafficking to the plasma membrane followed by internalization and transport to the ERC. We further show that internalization from the plasma membrane and delivery to the ERC are dependent on phosphorylation by Ca2+-dependent protein kinase Cα or β. As such, correct targeting of Syt IX is facilitated by the phorbol ester TPA but prevented by the cPKC inhibitor Go 6976.

Original languageEnglish
Pages (from-to)1641-1649
Number of pages9
JournalJournal of cell science
Volume118
Issue number8
DOIs
Publication statusPublished - 2005 Apr 15
Externally publishedYes

Keywords

  • Endocytic recycling compartment (ERC)
  • Endocytosis
  • Protein kinase C
  • RBL-2H3 mast cells
  • Synaptotagmin

ASJC Scopus subject areas

  • Cell Biology

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