Clarithromycin Dose-Dependently Stabilizes Rat Peritoneal Mast Cells

Itsuro Kazama, Kazutomo Saito, Asuka Baba, Tomohiro Mori, Nozomu Abe, Yasuhiro Endo, Hiroaki Toyama, Yutaka Ejima, Mitsunobu Matsubara, Masanori Yamauchi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Macrolides, such as clarithromycin, have antiallergic properties. Since exocytosis in mast cells is detected electrophysiologically via changes in membrane capacitance (Cm), the absence of such changes due to the drug indicates its mast cell-stabilizing effect. Methods: Employing the whole-cell patch clamp technique in rat peritoneal mast cells, we examined the effects of clarithromycin on Cm during exocytosis. Using a water-soluble fluorescent dye, we also examined its effect on deformation of the plasma membrane. Results: Clarithromycin (10 and 100 μM) significantly inhibited degranulation from mast cells and almost totally suppressed the GTP-γ-S-induced increase in Cm. It washed out the trapping of the dye on the surface of mast cells. Conclusions: This study provides for the first time electrophysiological evidence that clarithromycin dose-dependently inhibits the process of exocytosis. The mast cell-stabilizing action of clarithromycin may be attributable to its counteractive effect on plasma membrane deformation induced by exocytosis.

Original languageEnglish
Pages (from-to)295-303
Number of pages9
JournalChemotherapy
Volume61
Issue number6
DOIs
Publication statusPublished - 2016 Apr 13

Keywords

  • Clarithromycin
  • Exocytosis
  • Mast cell-stabilizing effect
  • Membrane capacitance
  • Plasma membrane deformation
  • Rat peritoneal mast cells

ASJC Scopus subject areas

  • Oncology
  • Pharmacology
  • Drug Discovery
  • Pharmacology (medical)
  • Infectious Diseases

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