TY - JOUR
T1 - Citral, an inhibitor of retinoic acid synthesis, modifies chick limb development
AU - Tanaka, Mikiko
AU - Tamura, Koji
AU - Ide, Hiroyuki
N1 - Funding Information:
We are grateful to Dr. J. P. Brockes for his critically reading and improving the manuscript and making valuable suggestions and comments. This work was supported by research grants from the Ministry of Education, Science and Culture of Japan.
PY - 1996/5/1
Y1 - 1996/5/1
N2 - Exogenously applied retinoic acid (RA) is known to affect cartilage pattern in developing and regenerating limbs. There are, however, few reports which analyze the participation of endogenous RA in limb pattern formation. Using an organ culture system, we attempted to reduce the concentration of endogenous RA in the developing chick wing buds by the treatment with citral (3,7-dimethyl-2,6-octadienal), an inhibitor of retinoic acid formation. After this treatment, the cultured wing buds were grafted to the stumps of host embryos. These citral-treated limb buds frequently formed truncated cartilage elements and the defects were rescued by simultaneous treatment with an appropriate concentration of RA. These results suggest that endogenous RA plays a role in chick limb bud development.
AB - Exogenously applied retinoic acid (RA) is known to affect cartilage pattern in developing and regenerating limbs. There are, however, few reports which analyze the participation of endogenous RA in limb pattern formation. Using an organ culture system, we attempted to reduce the concentration of endogenous RA in the developing chick wing buds by the treatment with citral (3,7-dimethyl-2,6-octadienal), an inhibitor of retinoic acid formation. After this treatment, the cultured wing buds were grafted to the stumps of host embryos. These citral-treated limb buds frequently formed truncated cartilage elements and the defects were rescued by simultaneous treatment with an appropriate concentration of RA. These results suggest that endogenous RA plays a role in chick limb bud development.
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U2 - 10.1006/dbio.1996.0111
DO - 10.1006/dbio.1996.0111
M3 - Article
C2 - 8626029
AN - SCOPUS:0029927334
VL - 175
SP - 239
EP - 247
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 2
ER -