Cis binding between inhibitory receptors and MHC class I can regulate mast cell activation

Ai Masuda, Akira Nakamura, Tsutomu Maeda, Yuzuru Sakamoto, Toshiyuki Takai

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Allergy is caused by immune effector cells, including mast cells and basophils. Cellular signaling that activates these effector cells is regulated by different inhibitory receptors on their surface. We show that human leukocyte immunoglobulin (Ig)-like receptor (LILR) B2 and its mouse orthologue, paired Ig-like receptor (PIR)-B, constitutively associate to major histocompatibility complex (MHC) class I on the same cell surface (in cis). The IgE-mediated effector responses were augmented in β2-microglobulin (β2m) and PIR-B-deficient mast cells. In addition, the increased cytokine production of β2m-deficient mast cells was not affected by the co-culture with MHC class I-positive mast cells, showing that less cis interaction between PIR-B and MHC class I on mast cells led to the increased cytokine release. Thus, the constitutive cis binding between LILRB2 or PIR-B and MHC class I has an essential role in regulating allergic responses. JEM

Original languageEnglish
Pages (from-to)907-920
Number of pages14
JournalJournal of Experimental Medicine
Volume204
Issue number4
DOIs
Publication statusPublished - 2007 Apr 16

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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