Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions

Satoru Nagatoishi, Yoshikazu Tanaka, Kouhei Tsumoto

Research output: Contribution to journalArticlepeer-review

124 Citations (Scopus)

Abstract

It is noteworthy that the formation of the DNA G-quadruplex is induced by factors other than stabilizing cations because this event probably occurs in living cells. Previous studies have shown that thrombin-binding DNA aptamer (TBA) forms a chair-type intramolecular G-quadruplex structure that binds with thrombin protein in the absence of stabilizing cations. Here, we used circular dichroism (CD) spectroscopy to confirm G-quadruplex formation in the presence of thrombin without stabilizing cations. We obtained characteristic CD spectra that demonstrated that TBA forms the distinctive G-quadruplex structure. Additionally, we investigated G-quadruplex formation induced by change of solvent environment: the influence of low-temperature conditions and molecular crowding.

Original languageEnglish
Pages (from-to)812-817
Number of pages6
JournalBiochemical and biophysical research communications
Volume352
Issue number3
DOIs
Publication statusPublished - 2007 Jan 19
Externally publishedYes

Keywords

  • Aptamer
  • Circular dichroism
  • DNA
  • G-quadruplex
  • Solvent

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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