Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2

Satoko Yoshiba, Hidetaka Shiratori, Ivana Y. Kuo, Aiko Kawasumi, Kyosuke Shinohara, Shigenori Nonaka, Yasuko Asai, Genta Sasaki, Jose Antonio Belo, Hiroshi Sasaki, Junichi Nakai, Bernd Dworniczak, Barbara E. Ehrlich, Petra Pennekamp, Hiroshi Hamada

Research output: Contribution to journalArticlepeer-review

182 Citations (Scopus)

Abstract

Unidirectional fluid flow plays an essential role in the breaking of left-right (L-R) symmetry in mouse embryos, but it has remained unclear how the flow is sensed by the embryo. We report that the Ca2+ channel Polycystin-2 (Pkd2) is required specifically in the perinodal crown cells for sensing the nodal flow. Examination of mutant forms of Pkd2 shows that the ciliary localization of Pkd2 is essential for correct L-R patterning. Whereas Kif3a mutant embryos, which lack all cilia, failed to respond to an artificial flow, restoration of primary cilia in crown cells rescued the response to the flow. Our results thus suggest that nodal flow is sensed in a manner dependent on Pkd2 by the cilia of crown cells located at the edge of the node.

Original languageEnglish
Pages (from-to)226-231
Number of pages6
JournalScience
Volume338
Issue number6104
DOIs
Publication statusPublished - 2012 Oct 12
Externally publishedYes

ASJC Scopus subject areas

  • General

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