TY - JOUR
T1 - Cigarette smoke augments the expression and responses of toll-like receptor 3 in human macrophages
AU - Koarai, Akira
AU - Yanagisawa, Satoru
AU - Sugiura, Hisatoshi
AU - Ichikawa, Tomohiro
AU - Akamatsu, Keiichiro
AU - Hirano, Tsunahiko
AU - Nakanishi, Masanori
AU - Matsunaga, Kazuto
AU - Minakata, Yoshiaki
AU - Ichinose, Masakazu
PY - 2012/8
Y1 - 2012/8
N2 - Background and objective: Cigarette smoking is the main risk factor for the development of chronic obstructive pulmonary disease (COPD). Recently, toll-like receptor 3 (TLR3) was shown to recognize pathogen-associated molecular patterns, especially viral-derived double-stranded RNA, and to be involved in immune responses. However, the effects of cigarette smoke on TLR3 remain unclear. In this study, it was examined whether cigarette smoke affects the expression and responses of TLR3 in human macrophages. Methods: The expression of TLR3 in alveolar macrophages from human lung tissues was analysed by immunohistochemistry, and the correlation of TLR3 expression with smoking history and lung function was evaluated. In addition, the effect of cigarette smoke on the expression and responses of TLR3 in macrophage lineage cells was investigated. Results: TLR3-positive alveolar macrophage numbers were significantly increased in smokers and COPD patients compared with non-smoking control subjects, but there was no difference between smokers and COPD patients. TLR3-positive macrophage numbers were positively correlated with smoking history and inversely correlated with corrected carbon monoxide diffusing capacity, but were not correlated with % predicted forced expiratory volume in 1 s. Furthermore, cigarette smoke extract potentiated the expression of TLR3 in monocyte-derived macrophages and significantly augmented the release of interleukin-8, as well as total matrix metalloproteinase-9 activity, in cells treated with TLR3 ligand. Conclusions: These data suggest that cigarette smoke augments the expression and responses of TLR3 in human macrophages, and this may contribute to neutrophilic airway inflammation and parenchymal destruction in the lungs of smokers and patients with COPD. The expression of TLR3 was increased in macrophages from smokers and COPD patients, and cigarette smoke potentiated the expression and responses of TLR3 in macrophage lineage cells. This may contribute to neutrophilic airway inflammation and parenchymal destruction in the lungs of smokers and COPD patients.
AB - Background and objective: Cigarette smoking is the main risk factor for the development of chronic obstructive pulmonary disease (COPD). Recently, toll-like receptor 3 (TLR3) was shown to recognize pathogen-associated molecular patterns, especially viral-derived double-stranded RNA, and to be involved in immune responses. However, the effects of cigarette smoke on TLR3 remain unclear. In this study, it was examined whether cigarette smoke affects the expression and responses of TLR3 in human macrophages. Methods: The expression of TLR3 in alveolar macrophages from human lung tissues was analysed by immunohistochemistry, and the correlation of TLR3 expression with smoking history and lung function was evaluated. In addition, the effect of cigarette smoke on the expression and responses of TLR3 in macrophage lineage cells was investigated. Results: TLR3-positive alveolar macrophage numbers were significantly increased in smokers and COPD patients compared with non-smoking control subjects, but there was no difference between smokers and COPD patients. TLR3-positive macrophage numbers were positively correlated with smoking history and inversely correlated with corrected carbon monoxide diffusing capacity, but were not correlated with % predicted forced expiratory volume in 1 s. Furthermore, cigarette smoke extract potentiated the expression of TLR3 in monocyte-derived macrophages and significantly augmented the release of interleukin-8, as well as total matrix metalloproteinase-9 activity, in cells treated with TLR3 ligand. Conclusions: These data suggest that cigarette smoke augments the expression and responses of TLR3 in human macrophages, and this may contribute to neutrophilic airway inflammation and parenchymal destruction in the lungs of smokers and patients with COPD. The expression of TLR3 was increased in macrophages from smokers and COPD patients, and cigarette smoke potentiated the expression and responses of TLR3 in macrophage lineage cells. This may contribute to neutrophilic airway inflammation and parenchymal destruction in the lungs of smokers and COPD patients.
KW - chronic obstructive pulmonary disease
KW - innate immunity
KW - metalloproteinase
KW - pulmonary diffusing capacity
KW - smoking
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U2 - 10.1111/j.1440-1843.2012.02198.x
DO - 10.1111/j.1440-1843.2012.02198.x
M3 - Article
C2 - 22591330
AN - SCOPUS:84864463796
VL - 17
SP - 1018
EP - 1025
JO - Respirology
JF - Respirology
SN - 1323-7799
IS - 6
ER -