Cidaroids, clypeasteroids, and spatangoids: Procurement, culture, and basic methods

Taku Hibino, Takuya Minokawa, Atsuko Yamazaki

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

This chapter describes practical methods and key points for using non-camarodont echinoids including cidaroids (Order Cidaroida), clypeasteroids (also known as sand dollars; Order Clypeasteroida), and spatangoids (also known as heart urchins; Order Spatangoida) as experimental subjects for biological studies. The content described here is based on six Japanese species of echinoids (Astriclypeus manni, Clypeaster japonicus, Echinocardium cordatum, Peronella japonica, Prionocidaris baculosa, and Scaphechinus mirabilis). Specific topics addressed in this chapter include the collection and maintenance of adults, embryonic culture, and experimental procedures for micromanipulations, whole mount in situ hybridization, and immunological experiments.

Original languageEnglish
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages81-103
Number of pages23
DOIs
Publication statusPublished - 2019 Jan 1

Publication series

NameMethods in Cell Biology
Volume150
ISSN (Print)0091-679X

Keywords

  • Cidaroida
  • Cidaroids
  • Clypeasteroida
  • Clypeasteroids
  • Immunology
  • Laboratory culture
  • Micromanipulation
  • Spatangoida
  • Spatangoids
  • WMISH

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Hibino, T., Minokawa, T., & Yamazaki, A. (2019). Cidaroids, clypeasteroids, and spatangoids: Procurement, culture, and basic methods. In Methods in Cell Biology (pp. 81-103). (Methods in Cell Biology; Vol. 150). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2018.09.012