Abstract
This study was designed to assess whether blocking endogenous endothelin with anti-endothelin antibodies could alter the development of hypertension in stroke-prone spontaneously hypertensive rats (SHR) and DOCA-salt treated rats. Specific anti-endothelin antibodies were produced in rabbits by standard methods. The amount of anti-endothelin antibodies employed in this study blocked the hypertensive effect of endothelin-1, 750ng/kg, by 55% in conscious rats. Intravenous injection of anti-endothelin antibodies as a bolus twice a week for 3 weeks did not affect the rise in blood pressure of stroke-prone SHR (268±8 mmHg, n=8) compared to control stroke-prone SHR (256±7 mmHg, n=8) treated with normal rabbit serum. Intravenous administration of anti-endothelin antibodies in a same manner also failed to alter the development of hypertension in DOCA-salt treated rats (160±6mmHg in anti-endothelin antibodies-treated group, n=7 compared to 164±5mmHg in normal rabbit serum-treated group, n=7). The administration of anti-endothelin antibodies did not induce any significant changes in body weight, urine volume and urinary sodium excretion in stroke-prone SHR and DOCA-salt treated rats compared to those treated with normal rabbit serum. These findings suggest that circulating endothelin might not play a major role in the regulation of blood pressure in stroke-prone SHR and DOCA-salt treated rats.
Original language | English |
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Pages (from-to) | 43-50 |
Number of pages | 8 |
Journal | Tohoku Journal of Experimental Medicine |
Volume | 169 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1993 |
Keywords
- DOCA-salt hypertensive rats
- anti-endothelin antibodies
- endothelin
- hypertension
- stroke-prone SHR
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)