Chromosomal assignment of human genes for gastrin, thyrotropin (TSH)-β subunit and C-erbB-2 by chromosome sorting combined with velocity sedimentation and southern hybridization

Shinichi Fukushige, Tomoaki Murotsu, Kenichi Matsubara

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Human genes for gastrin, thyrotropin (THS)-β subunit and c-erbB-2 were assigned to specific chromosomes using a single-laser cell sorter. For this purpose, condensed human chromosomes prepared from a karyotypically normal lymphoblastoid cell line were preliminarily fractionated by velocity sedimentation, and then sorted using a fluorescence-activated cell sorter. DNA was then extracted from the chromosomes, cleaved by restriction enzymes, and subjected to Southern hybridization using gene-specific radioactive probes. When the assignment of specific chromosomes was not possible due to chromosomal size overlapping, sorted chromosomes from cell lines carrying chromosomal translocation or from hybrid cells carrying known human chromosomes were used in addition. The results indicate that human genes for gastrin, TSH-β, and c-erbB-2 are located on chromosomes 17, 1 and 17, respectively.

Original languageEnglish
Pages (from-to)477-483
Number of pages7
JournalBiochemical and biophysical research communications
Volume134
Issue number2
DOIs
Publication statusPublished - 1986 Jan 29
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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