Chromatographic behavior of bile acids using cyclodextrin in mobile phase of high performance liquid chromatography

Kazutake Shimada, Yoshihiro Komine, Tomoyuki Oe

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The chromatographic behavior of bile acids was examined by the addition of cyclodextrin to the mobile phase of reversed-phase high performance liquid chromatography. The separation of bile acids was much improved by the addition of β-cyclodextrin. The capacity factors of bile acids having a 12 α-nydroxyl group were not so influenced but those of other bile acids were decreased sharply by the additives. The response of fluorescence labeled derivative of bile acid to a fluorescence detector was raised with an addition of cyclodextrin to the mobile phase.

Original languageEnglish
Pages (from-to)491-500
Number of pages10
JournalJournal of Liquid Chromatography
Volume12
Issue number4
DOIs
Publication statusPublished - 1989 Jan 3
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine

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