Abstract
The chromatographic behavior of bile acids was examined by the addition of cyclodextrin to the mobile phase of reversed-phase high performance liquid chromatography. The separation of bile acids was much improved by the addition of β-cyclodextrin. The capacity factors of bile acids having a 12 α-nydroxyl group were not so influenced but those of other bile acids were decreased sharply by the additives. The response of fluorescence labeled derivative of bile acid to a fluorescence detector was raised with an addition of cyclodextrin to the mobile phase.
| Original language | English |
|---|---|
| Pages (from-to) | 491-500 |
| Number of pages | 10 |
| Journal | Journal of Liquid Chromatography |
| Volume | 12 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1989 Jan 3 |
| Externally published | Yes |
ASJC Scopus subject areas
- Molecular Medicine