Cholesterol is more susceptible to oxidation than linoleates in cultured cells under oxidative stress induced by selenium deficiency and free radicals

Yoshiro Saito, Yasukazu Yoshida, Etsuo Niki

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Polyunsaturated fatty acids and their esters are known to be susceptible to free-radical mediated oxidation, while cholesterol is more resistant to oxidation. The present study focused on the relative susceptibilities of linoleates and cholesterol in Jurkat cells under oxidative stress induced by selenium deficiency and free radical insult, as assessed by total hydroxyoctadecadienoic acids (tHODE) and total 7-hydroxycholesterol (t7-OHCh) measured after reduction and saponification. It was observed that the levels of tHODE and t7-OHCh significantly increased by both oxidative insults. The increased amounts of t7-OHCh were higher than those of tHODE in both selenium-deficient and free radical-treated cells. These results suggest that, in contrast to plasma oxidation where cholesterol is much more resistant to oxidation than linoleates, cellular cholesterol is more susceptible to oxidation than cellular linoleates.

Original languageEnglish
Pages (from-to)4349-4354
Number of pages6
JournalFEBS Letters
Volume581
Issue number22
DOIs
Publication statusPublished - 2007 Sep 4

Keywords

  • Free radical
  • Lipid peroxidation
  • Oxidative stress
  • Selenium
  • Total 7-hydroxycholesterol
  • Total hydroxyoctadecadienoic acid

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Cholesterol is more susceptible to oxidation than linoleates in cultured cells under oxidative stress induced by selenium deficiency and free radicals'. Together they form a unique fingerprint.

Cite this