Cholesterol esterase bound to intestinal brush border membranes does not accelerate incorporation of micellar cholesterol into absorptive cells

Ikuo Ikeda, Kosuke Mitsui, Ryosuke Matsuoka, Tadateru Hamada, Sachiko Imabayashi, Akira Uchino, Koji Yamada, Katsumi Imaizumi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We confirmed that cholesterol esterase accelerated the incorporation of unesterified cholesterol solubilized in bile salt micelles into differentiated Caco-2 cells under various experimental conditions. Rat pancreatic juice and bovine cholesterol esterase increased the incorporation of micellar cholesterol into rat intestinal brush border membranes. The incorporation of micellar cholesterol was not changed in the brush border membranes enriched in and depleted of cholesterol esterase. The results suggest that the accelerated incorporation of micellar cholesterol by cholesterol esterase into absorptive cells is not mediated by the enzyme bound to the brush border membranes.

Original languageEnglish
Pages (from-to)2381-2387
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number11
DOIs
Publication statusPublished - 2003 Nov 1
Externally publishedYes

Keywords

  • Brush border membranes
  • Caco-2 cells
  • Cholesterol absorption
  • Cholesterol esterase
  • Micelles

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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