Cholesterol esterase bound to intestinal brush border membranes does not accelerate incorporation of micellar cholesterol into absorptive cells

Ikuo Ikeda; Kosuke Mitsui; Ryosuke Matsuoka; Tadateru Hamada; Sachiko Imabayashi; Akira Uchino; Koji Yamada; Katsumi Imaizumi

doi:10.1271/bbb.67.2381

Cholesterol esterase bound to intestinal brush border membranes does not accelerate incorporation of micellar cholesterol into absorptive cells

Ikuo Ikeda, Kosuke Mitsui, Ryosuke Matsuoka, Tadateru Hamada, Sachiko Imabayashi, Akira Uchino, Koji Yamada, Katsumi Imaizumi

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We confirmed that cholesterol esterase accelerated the incorporation of unesterified cholesterol solubilized in bile salt micelles into differentiated Caco-2 cells under various experimental conditions. Rat pancreatic juice and bovine cholesterol esterase increased the incorporation of micellar cholesterol into rat intestinal brush border membranes. The incorporation of micellar cholesterol was not changed in the brush border membranes enriched in and depleted of cholesterol esterase. The results suggest that the accelerated incorporation of micellar cholesterol by cholesterol esterase into absorptive cells is not mediated by the enzyme bound to the brush border membranes.

Original language	English
Pages (from-to)	2381-2387
Number of pages	7
Journal	Bioscience, Biotechnology and Biochemistry
Volume	67
Issue number	11
DOIs	https://doi.org/10.1271/bbb.67.2381
Publication status	Published - 2003 Nov 1
Externally published	Yes

Keywords

Brush border membranes
Caco-2 cells
Cholesterol absorption
Cholesterol esterase
Micelles

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.67.2381

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Cholesterol esterase bound to intestinal brush border membranes does not accelerate incorporation of micellar cholesterol into absorptive cells. / Ikeda, Ikuo; Mitsui, Kosuke; Matsuoka, Ryosuke; Hamada, Tadateru; Imabayashi, Sachiko; Uchino, Akira; Yamada, Koji; Imaizumi, Katsumi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 67, No. 11, 01.11.2003, p. 2381-2387.

Research output: Contribution to journal › Article › peer-review

Ikeda, Ikuo ; Mitsui, Kosuke ; Matsuoka, Ryosuke ; Hamada, Tadateru ; Imabayashi, Sachiko ; Uchino, Akira ; Yamada, Koji ; Imaizumi, Katsumi. / Cholesterol esterase bound to intestinal brush border membranes does not accelerate incorporation of micellar cholesterol into absorptive cells. In: Bioscience, Biotechnology and Biochemistry. 2003 ; Vol. 67, No. 11. pp. 2381-2387.

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abstract = "We confirmed that cholesterol esterase accelerated the incorporation of unesterified cholesterol solubilized in bile salt micelles into differentiated Caco-2 cells under various experimental conditions. Rat pancreatic juice and bovine cholesterol esterase increased the incorporation of micellar cholesterol into rat intestinal brush border membranes. The incorporation of micellar cholesterol was not changed in the brush border membranes enriched in and depleted of cholesterol esterase. The results suggest that the accelerated incorporation of micellar cholesterol by cholesterol esterase into absorptive cells is not mediated by the enzyme bound to the brush border membranes.",

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AU - Ikeda, Ikuo

AU - Mitsui, Kosuke

AU - Matsuoka, Ryosuke

AU - Hamada, Tadateru

AU - Imabayashi, Sachiko

AU - Uchino, Akira

AU - Yamada, Koji

AU - Imaizumi, Katsumi

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N2 - We confirmed that cholesterol esterase accelerated the incorporation of unesterified cholesterol solubilized in bile salt micelles into differentiated Caco-2 cells under various experimental conditions. Rat pancreatic juice and bovine cholesterol esterase increased the incorporation of micellar cholesterol into rat intestinal brush border membranes. The incorporation of micellar cholesterol was not changed in the brush border membranes enriched in and depleted of cholesterol esterase. The results suggest that the accelerated incorporation of micellar cholesterol by cholesterol esterase into absorptive cells is not mediated by the enzyme bound to the brush border membranes.

AB - We confirmed that cholesterol esterase accelerated the incorporation of unesterified cholesterol solubilized in bile salt micelles into differentiated Caco-2 cells under various experimental conditions. Rat pancreatic juice and bovine cholesterol esterase increased the incorporation of micellar cholesterol into rat intestinal brush border membranes. The incorporation of micellar cholesterol was not changed in the brush border membranes enriched in and depleted of cholesterol esterase. The results suggest that the accelerated incorporation of micellar cholesterol by cholesterol esterase into absorptive cells is not mediated by the enzyme bound to the brush border membranes.

KW - Brush border membranes

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KW - Cholesterol absorption

KW - Cholesterol esterase

KW - Micelles

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Cholesterol esterase bound to intestinal brush border membranes does not accelerate incorporation of micellar cholesterol into absorptive cells

Abstract

Keywords

ASJC Scopus subject areas

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