Cholesterol and plant sterol efflux from cultured intestinal epithelial cells is mediated by ATP-binding cassette transporters

Shizuko Tachibana, Maki Hirano, Takashi Hirata, Michinori Matsuo, Ikuo Ikeda, Kazumitsu Ueda, Ryuichiro Sato

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

In this study we analyzed functions of ATP-binding cassette (ABC) transporters involved in sterol transport from Caco-2 cells. Treatment with a synthetic liver x receptor ligand elevated both mRNA and protein levels of ABCG5, G8, and ABCA1. The ligand stimulated cholesterol efflux, suggesting that ABC transporters are involved in it. To identify the acceptors of cholesterol, potential molecules such as apolipoprotein A-I, glycocholic acid, phosphatidylcholine, and bile acid micelles were added to the medium. Apo A-I, a known acceptor of cholesterol transported by ABCA1, elevated cholesterol efflux on the basal side, whereas the others raised cholesterol efflux on the apical side. Moreover, bile acid micelles preferentially augmented plant sterol efflux rather than cholesterol. Finally, in HEK293 cells stably expressing ABCG5/G8, bile acid micelle-mediated sterol efflux was significantly accelerated. These results indicate that ABCG5/G8, unlike ABCA1, together with bile acids should participate in sterol efflux on the apical surface of Caco-2 cells.

Original languageEnglish
Pages (from-to)1886-1895
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume71
Issue number8
DOIs
Publication statusPublished - 2007

Keywords

  • ATP-binding cassette transporter G5/G8
  • Caco-2 cell
  • Cholesterol
  • Intestinal sterol excretion
  • Plant sterol

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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