ChlH, the H subunit of the Mg-chelatase, is an anti-sigma factor for SigE in Synechocystis sp. PCC 6803

Takashi Osanai, Masahiko Imashimizu, Asako Seki, Shusei Sato, Satoshi Tabata, Sousuke Imamura, Munehiko Asayama, Masahiko Ikeuchi, Kan Tanaka

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Although regulation of sigma factors has been intensively investigated, anti-sigma factors have not been identified in oxygenic photosynthetic organisms. A previous study suggested that the sigma factor, SigE, of the cyanobacterium Synechocystis sp. PCC 6803, a positive regulator of sugar catabolism, is posttranslation-ally activated by light-to-dark transition. In the present study, we found that the Hsubunit of Mg-chelataseChlH interacts with sigma factor SigE by yeast two-hybrid screening, and immunoprecipita-tion analysis revealed that ChlH associates with SigE in a light-dependent manner in vivo. We also found that Mg2+ promotes the interaction of SigE and ChlH and determines their localization in vitro. In vitro transcription analysis demonstrated that ChlH inhibits the transcription activity of SigE. Based on these results, we propose a model in which ChlH functions as an anti-sigma factor, transducing light signals to SigE in a process mediated by Mg 2+.

Original languageEnglish
Pages (from-to)6860-6865
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number16
DOIs
Publication statusPublished - 2009 Apr 21
Externally publishedYes

Keywords

  • Cyanobacteria
  • Transcription

ASJC Scopus subject areas

  • General

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