Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice

A. Okuzaki, Kinya Toriyama

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Site-specific mutagenesis in a rice genome was obtained by introducing chimeric RNA/DNA oligonucleotides (COs) by means of particle bombardment. Three COs were designed to target the independent codons for Pro-171, Trp-548 and Ser-627 of the endogenous rice acetolactate synthase (ALS) gene so it would confer resistance to ALS-inhibiting herbicides. Sequencing of the ALS gene of herbicide-resistant plants demonstrated that the ALS sequence was modified in a site-specific fashion. The efficiency of gene conversion mediated by COs was estimated to be 1x10-4. These results demonstrate that CO-directed gene targeting is feasible in rice.

Original languageEnglish
Pages (from-to)509-512
Number of pages4
JournalPlant Cell Reports
Volume22
Issue number7
DOIs
Publication statusPublished - 2004 Feb 1

Keywords

  • Acetolactate synthase
  • Chimeric RNA/DNA oligonucleotide
  • Gene targeting
  • Herbicide resistance
  • Oryza sativa L.

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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