Chimeric RNA Oligonucleotides with Triazole and Phosphate Linkages: Synthesis and RNA Interference

Tomoko Fujino, Kanako Kogashi, Koudai Okada, Martin Mattarella, Takeru Suzuki, Kenichi Yasumoto, Kazuhiro Sogawa, Hiroyuki Isobe

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Chimeric RNA oligonucleotides with an artificial triazole linker were synthesized using solution-phase click chemistry and solid-phase automated synthesis. Scalable synthesis methods for jointing units for the chimeric structure have been developed, and after click-coupling of the jointing units with triazole linkers, a series of chimeric oligonucleotides was prepared by utilizing the well-established phosphoramidite method for the elongation. The series of chimeric 21-mer oligonucleotides that possessed the triazole linker at different strands and positions allowed for a screening study of the RNA interference to clarify the preference of the triazole modifications in small-interfering RNA molecules.

    Original languageEnglish
    Pages (from-to)2683-2688
    Number of pages6
    JournalChemistry - An Asian Journal
    Volume10
    Issue number12
    DOIs
    Publication statusPublished - 2015 Dec 1

    Keywords

    • RNA
    • click chemistry
    • oligonucleotides
    • siRNA
    • solid-phase synthesis

    ASJC Scopus subject areas

    • Biochemistry
    • Organic Chemistry

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