Abstract
Chimeric RNA oligonucleotides with an artificial triazole linker were synthesized using solution-phase click chemistry and solid-phase automated synthesis. Scalable synthesis methods for jointing units for the chimeric structure have been developed, and after click-coupling of the jointing units with triazole linkers, a series of chimeric oligonucleotides was prepared by utilizing the well-established phosphoramidite method for the elongation. The series of chimeric 21-mer oligonucleotides that possessed the triazole linker at different strands and positions allowed for a screening study of the RNA interference to clarify the preference of the triazole modifications in small-interfering RNA molecules.
Original language | English |
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Pages (from-to) | 2683-2688 |
Number of pages | 6 |
Journal | Chemistry - An Asian Journal |
Volume | 10 |
Issue number | 12 |
DOIs | |
Publication status | Published - 2015 Dec 1 |
Keywords
- RNA
- click chemistry
- oligonucleotides
- siRNA
- solid-phase synthesis
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry