Chimeric RNA Oligonucleotides with Triazole and Phosphate Linkages: Synthesis and RNA Interference

Tomoko Fujino; Kanako Kogashi; Koudai Okada; Martin Mattarella; Takeru Suzuki; Kenichi Yasumoto; Kazuhiro Sogawa; Hiroyuki Isobe

doi:10.1002/asia.201500765

Chimeric RNA Oligonucleotides with Triazole and Phosphate Linkages: Synthesis and RNA Interference

Tomoko Fujino, Kanako Kogashi, Koudai Okada, Martin Mattarella, Takeru Suzuki, Kenichi Yasumoto, Kazuhiro Sogawa, Hiroyuki Isobe

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Chimeric RNA oligonucleotides with an artificial triazole linker were synthesized using solution-phase click chemistry and solid-phase automated synthesis. Scalable synthesis methods for jointing units for the chimeric structure have been developed, and after click-coupling of the jointing units with triazole linkers, a series of chimeric oligonucleotides was prepared by utilizing the well-established phosphoramidite method for the elongation. The series of chimeric 21-mer oligonucleotides that possessed the triazole linker at different strands and positions allowed for a screening study of the RNA interference to clarify the preference of the triazole modifications in small-interfering RNA molecules.

Original language	English
Pages (from-to)	2683-2688
Number of pages	6
Journal	Chemistry - An Asian Journal
Volume	10
Issue number	12
DOIs	https://doi.org/10.1002/asia.201500765
Publication status	Published - 2015 Dec 1

Keywords

RNA
click chemistry
oligonucleotides
siRNA
solid-phase synthesis

ASJC Scopus subject areas

Biochemistry
Organic Chemistry

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10.1002/asia.201500765

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Chimeric RNA Oligonucleotides with Triazole and Phosphate Linkages : Synthesis and RNA Interference. / Fujino, Tomoko; Kogashi, Kanako; Okada, Koudai; Mattarella, Martin; Suzuki, Takeru; Yasumoto, Kenichi; Sogawa, Kazuhiro; Isobe, Hiroyuki.

In: Chemistry - An Asian Journal, Vol. 10, No. 12, 01.12.2015, p. 2683-2688.

Research output: Contribution to journal › Article › peer-review

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AU - Mattarella, Martin

AU - Suzuki, Takeru

AU - Yasumoto, Kenichi

AU - Sogawa, Kazuhiro

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Chimeric RNA Oligonucleotides with Triazole and Phosphate Linkages: Synthesis and RNA Interference

Abstract

Keywords

ASJC Scopus subject areas

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