TY - JOUR
T1 - Chick derived induced pluripotent stem cells by the poly-cistronic transposon with enhanced transcriptional activity
AU - Katayama, Masafumi
AU - Hirayama, Takashi
AU - Tani, Tetsuya
AU - Nishimori, Katsuhiko
AU - Onuma, Manabu
AU - Fukuda, Tomokazu
N1 - Funding Information:
We thank Mr. Kenichiro Donai (Tohoku University), and Dr. Kengo Kuroda (Tohoku University) for helpful discussions. MK and TF designed the experiments. MK and TF wrote the manuscript. MK and TH performed experiments (MK: all data, TH: Figure c). MK analyzed date. TT, KN, and MO provided technical assistance. All authors discussed the results and commented on the manuscript. The authors declare that they have no conflict to interest.
Publisher Copyright:
© 2017 Wiley Periodicals, Inc.
PY - 2018/2
Y1 - 2018/2
N2 - Induced pluripotent stem (iPS) cell technology lead terminally differentiated cells into the pluripotent stem cells through the expression of defined reprogramming factors. Although, iPS cells have been established in a number of mammalian species, including mouse, human, and monkey, studies on iPS cells in avian species are still very limited. To establish chick iPS cells, six factors were used within the poly-cistronic reprogramming vector (PB-R6F), containing M3O (MyoD derived transactivation domain fused with Oct3/4), Sox2, Klf4, c-Myc, Lin28, and Nanog. The PB-R6F derived iPS cells were alkaline-phosphatase and SSEA-1 positive, which are markers of pluripotency. Elevated levels of endogenous Oct3/4 and Nanog genes were detected in the established iPS cells, suggesting the activation of the FGF signaling pathway is critical for the pluripotent status. Histological analysis of teratoma revealed that the established chick iPS cells have differentiation ability into three-germ-layer derived tissues. This is the first report of establishment of avian derived iPS cells with a single poly-cistronic transposon based expression system. The establishment of avian derived iPS cells could contribute to the genetic conservation and modification of avian species.
AB - Induced pluripotent stem (iPS) cell technology lead terminally differentiated cells into the pluripotent stem cells through the expression of defined reprogramming factors. Although, iPS cells have been established in a number of mammalian species, including mouse, human, and monkey, studies on iPS cells in avian species are still very limited. To establish chick iPS cells, six factors were used within the poly-cistronic reprogramming vector (PB-R6F), containing M3O (MyoD derived transactivation domain fused with Oct3/4), Sox2, Klf4, c-Myc, Lin28, and Nanog. The PB-R6F derived iPS cells were alkaline-phosphatase and SSEA-1 positive, which are markers of pluripotency. Elevated levels of endogenous Oct3/4 and Nanog genes were detected in the established iPS cells, suggesting the activation of the FGF signaling pathway is critical for the pluripotent status. Histological analysis of teratoma revealed that the established chick iPS cells have differentiation ability into three-germ-layer derived tissues. This is the first report of establishment of avian derived iPS cells with a single poly-cistronic transposon based expression system. The establishment of avian derived iPS cells could contribute to the genetic conservation and modification of avian species.
KW - avian
KW - induced pluripotent stem (iPS) cells
KW - pluripotent network
KW - primed type stem cell
UR - http://www.scopus.com/inward/record.url?scp=85019848940&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019848940&partnerID=8YFLogxK
U2 - 10.1002/jcp.25947
DO - 10.1002/jcp.25947
M3 - Article
C2 - 28387938
AN - SCOPUS:85019848940
SN - 0021-9541
VL - 233
SP - 990
EP - 1004
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 2
ER -