Chick derived induced pluripotent stem cells by the poly-cistronic transposon with enhanced transcriptional activity

Masafumi Katayama, Takashi Hirayama, Tetsuya Tani, Katsuhiko Nishimori, Manabu Onuma, Tomokazu Fukuda

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Induced pluripotent stem (iPS) cell technology lead terminally differentiated cells into the pluripotent stem cells through the expression of defined reprogramming factors. Although, iPS cells have been established in a number of mammalian species, including mouse, human, and monkey, studies on iPS cells in avian species are still very limited. To establish chick iPS cells, six factors were used within the poly-cistronic reprogramming vector (PB-R6F), containing M3O (MyoD derived transactivation domain fused with Oct3/4), Sox2, Klf4, c-Myc, Lin28, and Nanog. The PB-R6F derived iPS cells were alkaline-phosphatase and SSEA-1 positive, which are markers of pluripotency. Elevated levels of endogenous Oct3/4 and Nanog genes were detected in the established iPS cells, suggesting the activation of the FGF signaling pathway is critical for the pluripotent status. Histological analysis of teratoma revealed that the established chick iPS cells have differentiation ability into three-germ-layer derived tissues. This is the first report of establishment of avian derived iPS cells with a single poly-cistronic transposon based expression system. The establishment of avian derived iPS cells could contribute to the genetic conservation and modification of avian species.

Original languageEnglish
Pages (from-to)990-1004
Number of pages15
JournalJournal of Cellular Physiology
Volume233
Issue number2
DOIs
Publication statusPublished - 2018 Feb 1

Keywords

  • avian
  • induced pluripotent stem (iPS) cells
  • pluripotent network
  • primed type stem cell

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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