Chemistry-based RNA technologies: demonstration of usefulness of libraries of ribozymes and short hairpin RNAs (shRNAs).

Makoto Miyagishi, Sahohime Matsumoto, Hideo Akashi, Hiroaki Kawasaki, Taro Fukao, Yoko Fukuda, Masayuki Sano, Yoshio Kato, Yasuomi Takagi, Yoshiyuki Tanaka, Masaki Warashina, Tomoko Kuwabara, Shinya Y. Sawata, Yutaka Ikeda, Shun ichi Kawahara, Kaul C. Sunil, Renu Wadhwa, Kazunari Taira

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Mechanism of action of hammerhead ribozymes has been investigated and their intracellular activities have been improved. Based on the improved ribozymes and more recently discovered natural RNAi, we have created libraries of both ribozymes and short hairpin RNAs (shRNAs). The introduction of a library of active ribozymes or shRNAs into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function.

Original languageEnglish
Pages (from-to)91-92
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number49
DOIs
Publication statusPublished - 2005

ASJC Scopus subject areas

  • Medicine(all)

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