Chemiluminescence imaging of localized enzymes by scanning chemiluminescence microscopy

Shigenobu Kasai, Huafang Zhou, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A novel scanning chemiluminescence microscopy (SCLM) was used for imaging localized horse radish peroxidase (HRP) or glucose oxidase (GOD) immobilized at a solid substrate. SCLM equips a scanning capillary tip (diameter, 1 μm) for injecting a small amount of luminol onto the substrate to generate localized chemiluminescence. The chemiluminescense induced by the enzyme-catalyzed reaction was detected with a photon-counter. Two-demensional mapping of the photon-counting intensity against the tip position gave images indicating the activity of immobilized HRP and GOD.

Original languageEnglish
Pages (from-to)200-201
Number of pages2
JournalChemistry Letters
Issue number3
DOIs
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Chemistry(all)

Fingerprint

Dive into the research topics of 'Chemiluminescence imaging of localized enzymes by scanning chemiluminescence microscopy'. Together they form a unique fingerprint.

Cite this