Chemiluminescence-high performance liquid chromatography of phosphatidylcholine hydroperoxide

Teruo Miyazawa, Keiichi Yasuda, Kenshiro Fujimoto

Research output: Contribution to journalArticlepeer-review

155 Citations (Scopus)

Abstract

A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was developed for the hydroperoxide-specific detection of phosphatidylcholine hydroperoxide. In this system, chemiluminescence detector was equipped with normal phase HPLC. Luminol-cytochrome c solution was used as the hydroperoxide-specific luminescent reagent. A detection limit of 7 nmol hydroperoxide-02 of phosphatidylcholine hydroperoxide could be achieved.

Original languageEnglish
Pages (from-to)915-925
Number of pages11
JournalAnalytical Letters
Volume20
Issue number6
DOIs
Publication statusPublished - 1987 Jan 1

Keywords

  • HPLC
  • chemiluminescence
  • hydroperoxide
  • phosphatidylcholine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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