Chemiluminescence detection of 8a-hydroperoxy-tocopherone in photooxidized α-tocopherol

Teruo Miyazawa, Toyonobu Yamashita, Kenshiro Fujimoto

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Photosensitized oxidation products of α-tocopherol were analyzed by reversed phase high-performance liquid chromatography (CL-HPLC) in combination with chemiluminescence detection, to measure hydroperoxide derivatives. An intense peak was observed on the chemiluminescence chromatogram, corresponding to 8a-hydroperoxy-α-tocopherone (α-tocopherol hydroperoxide), the identity of which was confirmed by1H nuclear magnetic resonance and mass spectrometry. Observed peak intensities correlated with the peroxide value of oxidized α-tocopherol. As little as 50 pmol of 8a-hydroperoxy-α-tocopherone was detectable. The CL-HPLC method, which is specific for the detection of hydroperoxides, should prove useful in studies on tocopherol oxidation in foods and biological systems.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
Issue number4
Publication statusPublished - 1992 Apr 1

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology


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