Chemiluminescence detection of 8a-hydroperoxy-tocopherone in photooxidized α-tocopherol

Teruo Miyazawa; Toyonobu Yamashita; Kenshiro Fujimoto

doi:10.1007/BF02536478

Chemiluminescence detection of 8a-hydroperoxy-tocopherone in photooxidized α-tocopherol

Teruo Miyazawa, Toyonobu Yamashita, Kenshiro Fujimoto

New Industry Creation Hatchery Center (NICHe)

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Photosensitized oxidation products of α-tocopherol were analyzed by reversed phase high-performance liquid chromatography (CL-HPLC) in combination with chemiluminescence detection, to measure hydroperoxide derivatives. An intense peak was observed on the chemiluminescence chromatogram, corresponding to 8a-hydroperoxy-α-tocopherone (α-tocopherol hydroperoxide), the identity of which was confirmed by¹H nuclear magnetic resonance and mass spectrometry. Observed peak intensities correlated with the peroxide value of oxidized α-tocopherol. As little as 50 pmol of 8a-hydroperoxy-α-tocopherone was detectable. The CL-HPLC method, which is specific for the detection of hydroperoxides, should prove useful in studies on tocopherol oxidation in foods and biological systems.

Original language	English
Pages (from-to)	289-294
Number of pages	6
Journal	Lipids
Volume	27
Issue number	4
DOIs	https://doi.org/10.1007/BF02536478
Publication status	Published - 1992 Apr 1

ASJC Scopus subject areas

Biochemistry
Organic Chemistry
Cell Biology

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title = "Chemiluminescence detection of 8a-hydroperoxy-tocopherone in photooxidized α-tocopherol",

abstract = "Photosensitized oxidation products of α-tocopherol were analyzed by reversed phase high-performance liquid chromatography (CL-HPLC) in combination with chemiluminescence detection, to measure hydroperoxide derivatives. An intense peak was observed on the chemiluminescence chromatogram, corresponding to 8a-hydroperoxy-α-tocopherone (α-tocopherol hydroperoxide), the identity of which was confirmed by1H nuclear magnetic resonance and mass spectrometry. Observed peak intensities correlated with the peroxide value of oxidized α-tocopherol. As little as 50 pmol of 8a-hydroperoxy-α-tocopherone was detectable. The CL-HPLC method, which is specific for the detection of hydroperoxides, should prove useful in studies on tocopherol oxidation in foods and biological systems.",

author = "Teruo Miyazawa and Toyonobu Yamashita and Kenshiro Fujimoto",

year = "1992",

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journal = "Lipids",

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T1 - Chemiluminescence detection of 8a-hydroperoxy-tocopherone in photooxidized α-tocopherol

AU - Miyazawa, Teruo

AU - Yamashita, Toyonobu

AU - Fujimoto, Kenshiro

PY - 1992/4/1

Y1 - 1992/4/1

N2 - Photosensitized oxidation products of α-tocopherol were analyzed by reversed phase high-performance liquid chromatography (CL-HPLC) in combination with chemiluminescence detection, to measure hydroperoxide derivatives. An intense peak was observed on the chemiluminescence chromatogram, corresponding to 8a-hydroperoxy-α-tocopherone (α-tocopherol hydroperoxide), the identity of which was confirmed by1H nuclear magnetic resonance and mass spectrometry. Observed peak intensities correlated with the peroxide value of oxidized α-tocopherol. As little as 50 pmol of 8a-hydroperoxy-α-tocopherone was detectable. The CL-HPLC method, which is specific for the detection of hydroperoxides, should prove useful in studies on tocopherol oxidation in foods and biological systems.

AB - Photosensitized oxidation products of α-tocopherol were analyzed by reversed phase high-performance liquid chromatography (CL-HPLC) in combination with chemiluminescence detection, to measure hydroperoxide derivatives. An intense peak was observed on the chemiluminescence chromatogram, corresponding to 8a-hydroperoxy-α-tocopherone (α-tocopherol hydroperoxide), the identity of which was confirmed by1H nuclear magnetic resonance and mass spectrometry. Observed peak intensities correlated with the peroxide value of oxidized α-tocopherol. As little as 50 pmol of 8a-hydroperoxy-α-tocopherone was detectable. The CL-HPLC method, which is specific for the detection of hydroperoxides, should prove useful in studies on tocopherol oxidation in foods and biological systems.

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