Chemical structure of neutral sugar chains isolated from human mature milk κ-casein

Tadao Saito, Takatoshi Itoh, Susumu Adachi

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11 Citations (Scopus)

Abstract

The carbohydrate chains linked to human κ-casein from mature milk were released by alkaline borohydride treatment as reduced oligosaccharides. The neutral oligosaccharides of lower molecular weight were fractionated and purified by gel filtration and preparative thin layer chromatographies. Seven neutral oligosaccharides (a di- (0.5%), two tetra- (30.5%), two penta- (5.4%) and teo hexasaccharide alditols (10.9%)) were obtained in homogeneity, and followed by methylation analysis with gas-liquid chromatography-mass spectrometry and by anomer analysis with 13C nuclear magnetic resonance. Their chemical structures were identified to be Galβ1 → 3GalNAc-ol (I), Galβ1 → 3[Galβ1 → 4GlcNAcβ1 → 6]GalNAc-ol (II), Galβ1 → 3 [Fucα1 → 4GlcNAcβ1 → 6]GalNAc-ol (III), GlcNAcβ1 → 3 / 6Galβ1 → 3[Galβ1 → 4GlcNAcβ1 → 6] GalNAc-ol (IV), GlcNAcβ1 → 3 / 6Galβ1 → 3[Fucα1 → 4GlcNAcβ1 → 6]GalNAc-ol (V), Fucα1 → 4GlcNAcβ1 → 3 / 6Galβ1 → 3[Galβ1 → 4GlcNAcβ1 → 6]GalNAc-ol (VI) and Fucα1 → 4GlcNAcβ1 → 3 / 6Galβ1 → 3[Fucα1 → 4GlcNAcβ1 → 6]GalNAc-ol (VII). Five oligosaccharide alditols (III-VII) were the novel carbohydrate chains of κ-casein from mammalian milk.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalBBA - General Subjects
Volume964
Issue number2
DOIs
Publication statusPublished - 1988 Feb 17

Keywords

  • (Human milk)
  • Carbohydrate structure
  • Caseinoglycopeptide
  • NMR, C-
  • Neutral sugar chain
  • κ-Casein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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