Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system

Jung Min Yoon, Masatoshi Nakajima, Kiyoshi Mashiguchi, Seung Hyun Park, Masato Otani, Tadao Asami

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    We applied a yeast two-hybrid (Y2H) system to the high-throughput monitoring of two proteins' interaction, a receptor for phytohormone gibberellin (GA) and its direct signal transducer DELLA. With this system, we screened inhibitors to the interaction. As a result, we discovered a chemical, 3-(2-thienylsulfonyl)pyrazine-2-carbonitrile (TSPC), and we confirmed that TSPC is an inhibitor for GA perception by in vitro and in planta evaluations.

    Original languageEnglish
    Pages (from-to)1096-1098
    Number of pages3
    JournalBioorganic and Medicinal Chemistry Letters
    Volume23
    Issue number4
    DOIs
    Publication statusPublished - 2013 Feb 15

    Keywords

    • Gibberellin
    • Inhibitor
    • Receptor
    • Screening
    • Y2H

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Pharmaceutical Science
    • Drug Discovery
    • Clinical Biochemistry
    • Organic Chemistry

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