TY - JOUR
T1 - Chemical characterization of the oligosaccharides in milk of high Arctic harbour seal (Phoca vitulina vitulina)
AU - Urashima, Tadasu
AU - Nakamura, Tadashi
AU - Yamaguchi, Kohsuke
AU - Munakata, Jiro
AU - Arai, Ikichi
AU - Saito, Tadao
AU - Lydersen, Christian
AU - Kovacs, Kit M.
N1 - Funding Information:
We thank Dr Michael Messer of Department of Biochemistry, Department of Biochemistry, for the useful discussion. The National Research Council of Norway, in co-operation with the Norwegian Polar Institute, financed collection of the harbour seal milk. This study was partially supported by a Grant-in-Aid for Scientific Research (B) in Japan, grant number 13575026 and also by a grant from the 21st Century COE Program (A-1), Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2003/8
Y1 - 2003/8
N2 - Carbohydrates were extracted from high Arctic harbour seal milk, Phoca vitulina vitulina (family Phocidae). Free neutral oligosaccharides were separated by gel filtration and preparative thin layer chromatography, while free sialyl oligosaccharides were separated by gel filtration and then purified by ion exchange chromatography, gel filtration and high performance liquid chromatography. Oligosaccharide structures were determined by 1H-NMR spectroscopy. The structures of the neutral oligosaccharides were as follows: lactose, 2′-fucosyllactose, lacto-N-neotetraose, lacto-N-neohexaose, monofucosyl lacto-N-neohexaose and difucosyl lacto-N-neohexaose. Thus, all of the neutral saccharides contained lactose or lacto-N-neotetraose or lacto-N-neohexaose as core units and/or non-reducing α(1-2) linked fucose. These oligosaccharides have also been found in hooded seal milk. The structures of the silalyl oligosaccharides were: monosialyl lacto-N-neohexaose, monosialyl monofucosyl lacto-N-neohexaose, monosialyl difucosyl lacto-N-neohexaose and disialyl lacto-N-neohexaose. These oligosaccharides contained lacto-N-neohexaose as core units, and one or two α(2-6) linked Neu5Ac, and/or non-reducing α(1-2) linked Fuc. The Neu5Ac residues were found to be linked to GlcNAc or penultimate Gal residues. The acidic oligosaccharides are the first to have been characterized in the milk of any species of seal.
AB - Carbohydrates were extracted from high Arctic harbour seal milk, Phoca vitulina vitulina (family Phocidae). Free neutral oligosaccharides were separated by gel filtration and preparative thin layer chromatography, while free sialyl oligosaccharides were separated by gel filtration and then purified by ion exchange chromatography, gel filtration and high performance liquid chromatography. Oligosaccharide structures were determined by 1H-NMR spectroscopy. The structures of the neutral oligosaccharides were as follows: lactose, 2′-fucosyllactose, lacto-N-neotetraose, lacto-N-neohexaose, monofucosyl lacto-N-neohexaose and difucosyl lacto-N-neohexaose. Thus, all of the neutral saccharides contained lactose or lacto-N-neotetraose or lacto-N-neohexaose as core units and/or non-reducing α(1-2) linked fucose. These oligosaccharides have also been found in hooded seal milk. The structures of the silalyl oligosaccharides were: monosialyl lacto-N-neohexaose, monosialyl monofucosyl lacto-N-neohexaose, monosialyl difucosyl lacto-N-neohexaose and disialyl lacto-N-neohexaose. These oligosaccharides contained lacto-N-neohexaose as core units, and one or two α(2-6) linked Neu5Ac, and/or non-reducing α(1-2) linked Fuc. The Neu5Ac residues were found to be linked to GlcNAc or penultimate Gal residues. The acidic oligosaccharides are the first to have been characterized in the milk of any species of seal.
KW - Harbour seal milk
KW - Milk oligosaccharide
KW - Neutral oligosaccharide
KW - Phoca vitulina vitulina
KW - Phocidae
KW - Sialyl oligosaccharide
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U2 - 10.1016/S1095-6433(03)00130-2
DO - 10.1016/S1095-6433(03)00130-2
M3 - Article
C2 - 12890545
AN - SCOPUS:0142075385
VL - 135
SP - 549
EP - 563
JO - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
SN - 1095-6433
IS - 4
ER -