TY - JOUR
T1 - Chemical characterization of the oligosaccharides in Bactrian camel (Camelus bactrianus) milk and colostrum
AU - Fukuda, K.
AU - Yamamoto, A.
AU - Ganzorig, K.
AU - Khuukhenbaatar, J.
AU - Senda, A.
AU - Saito, T.
AU - Urashima, T.
N1 - Funding Information:
This study was partially supported by funding from the Global COE Program “Frontier Program for Animal Global Health and Hygiene” , Ministry of Education, Culture, Sports, Science, and Technology, Japan, from the Research and Development Program for New Bio-industry Initiatives, the Bio-oriented Technology Research Advancement Institution (BRAIN), National Agriculture and Food Research Organization (NARO, Saitama, Japan), and from Yotsuba Milk Products Co (Sapporo, Hokkaido, Japan).
PY - 2010/12
Y1 - 2010/12
N2 - Bactrian camel milk and colostrum are commonly used as foods in Mongolia, whose people believe that these products promote human health. It has been hypothesized that milk oligosaccharides are biologically significant components of human milk, acting as receptor analogs that inhibit the attachment of pathogenic microorganisms to the colonic mucosa, and as prebiotics, which stimulate the growth of bifidobacteria within the infant colon. To evaluate their biological significance, we studied the oligosaccharides present in samples of Bactrian camel milk and colostrum. Using 1H-nuclear magnetic resonance spectroscopy, we identified and characterized the following oligosaccharides of camel colostrum: Gal(β1-4)[Fuc(α1-3)]Glc (3-fucosyllactose), Gal(β1-3)Gal(β1-4)Glc (3′-galactosyllactose), Gal(β1-6)Gal(β1-4)Glc (6′-galactosyllactose), Neu5Ac(α2-3)Gal(β1-4)Glc (3′-sialyllactose), Neu5Ac(α2-6)Gal(β1-4)Glc (6′-sialyllactose), Neu5Ac(α2-3)Gal(β1-3)Gal(β1-4)Glc (sialyl-3′-galactosyllactose), Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (sialyllacto-N-tetraose c), Neu5Ac(α2-3)Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (sialyllacto-N-novopentaose a), Gal(β1-3)[Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (sialyllacto-N-novopentaose b); and Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (monosialyllacto-N-neohexaose). The oligosaccharides in the mature camel milk were characterized as 3′-galactosyllactose, Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (lacto-N-novopentaose I), and 3′-sialyllactose.
AB - Bactrian camel milk and colostrum are commonly used as foods in Mongolia, whose people believe that these products promote human health. It has been hypothesized that milk oligosaccharides are biologically significant components of human milk, acting as receptor analogs that inhibit the attachment of pathogenic microorganisms to the colonic mucosa, and as prebiotics, which stimulate the growth of bifidobacteria within the infant colon. To evaluate their biological significance, we studied the oligosaccharides present in samples of Bactrian camel milk and colostrum. Using 1H-nuclear magnetic resonance spectroscopy, we identified and characterized the following oligosaccharides of camel colostrum: Gal(β1-4)[Fuc(α1-3)]Glc (3-fucosyllactose), Gal(β1-3)Gal(β1-4)Glc (3′-galactosyllactose), Gal(β1-6)Gal(β1-4)Glc (6′-galactosyllactose), Neu5Ac(α2-3)Gal(β1-4)Glc (3′-sialyllactose), Neu5Ac(α2-6)Gal(β1-4)Glc (6′-sialyllactose), Neu5Ac(α2-3)Gal(β1-3)Gal(β1-4)Glc (sialyl-3′-galactosyllactose), Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (sialyllacto-N-tetraose c), Neu5Ac(α2-3)Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (sialyllacto-N-novopentaose a), Gal(β1-3)[Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (sialyllacto-N-novopentaose b); and Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (monosialyllacto-N-neohexaose). The oligosaccharides in the mature camel milk were characterized as 3′-galactosyllactose, Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (lacto-N-novopentaose I), and 3′-sialyllactose.
KW - Bactrian camel
KW - Colostrum
KW - Milk oligosaccharide
KW - Sialyl oligosaccharide
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U2 - 10.3168/jds.2010-3151
DO - 10.3168/jds.2010-3151
M3 - Article
C2 - 21094729
AN - SCOPUS:78649306009
VL - 93
SP - 5572
EP - 5587
JO - Journal of Dairy Science
JF - Journal of Dairy Science
SN - 0022-0302
IS - 12
ER -
