Chemical characterization of sialyl oligosaccharides isolated from tammar wallaby (Macropus eugenii) milk

Tadasu Urashima; Tadao Saito; Yuhnagi Tsuji; Yasuko Taneda; Toshihide Takasawa; Michael Messer

doi:10.1016/0304-4165(94)90028-0

Chemical characterization of sialyl oligosaccharides isolated from tammar wallaby (Macropus eugenii) milk

Tadasu Urashima, Tadao Saito, Yuhnagi Tsuji, Yasuko Taneda, Toshihide Takasawa, Michael Messer

AGR - Bioscience and Biotechnology for Future Bioindustries

Research output: Contribution to journal › Article

23 Citations (Scopus)

Abstract

Two components of the sialyl oligosaccharides were separated from milk of the tammar wallaby (Macropus eugenii) by gel filtration and ion exchange chromatography. Their molecular weights, estimated by gel filtration on HPLC using 5 mM triethylamine-acetate buffer (pH 5.0), were approx. 3,000. Their monosaccharide compositions, determined by GC analysis after methanolysis and by colorimetric assay, were (Glc)₁(Gal)₉(GlcNAc)₂(Neu5Ac)₁, and (Glc)₁(Gal)₈(GlcNAc)₂(Neu5Ac)₂. Their chemical structures were further elucidated by ¹H-NMR and methylation analysis. The results suggest that their approximate structures are: {A figure is presented}.

Original language	English
Pages (from-to)	64-72
Number of pages	9
Journal	BBA - General Subjects
Volume	1200
Issue number	1
DOIs	https://doi.org/10.1016/0304-4165(94)90028-0
Publication status	Published - 1994 May 25

Keywords

(M. eugenii)
Higher Oligosaccharide
Marsupial
Milk
NMR-H
Sialyl Oligosaccharide

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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Urashima, T., Saito, T., Tsuji, Y., Taneda, Y., Takasawa, T., & Messer, M. (1994). Chemical characterization of sialyl oligosaccharides isolated from tammar wallaby (Macropus eugenii) milk. BBA - General Subjects, 1200(1), 64-72. https://doi.org/10.1016/0304-4165(94)90028-0

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abstract = "Two components of the sialyl oligosaccharides were separated from milk of the tammar wallaby (Macropus eugenii) by gel filtration and ion exchange chromatography. Their molecular weights, estimated by gel filtration on HPLC using 5 mM triethylamine-acetate buffer (pH 5.0), were approx. 3,000. Their monosaccharide compositions, determined by GC analysis after methanolysis and by colorimetric assay, were (Glc)1(Gal)9(GlcNAc)2(Neu5Ac)1, and (Glc)1(Gal)8(GlcNAc)2(Neu5Ac)2. Their chemical structures were further elucidated by 1H-NMR and methylation analysis. The results suggest that their approximate structures are: {A figure is presented}.",

keywords = "(M. eugenii), Higher Oligosaccharide, Marsupial, Milk, NMR-H, Sialyl Oligosaccharide",

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AU - Urashima, Tadasu

AU - Saito, Tadao

AU - Tsuji, Yuhnagi

AU - Taneda, Yasuko

AU - Takasawa, Toshihide

AU - Messer, Michael

PY - 1994/5/25

Y1 - 1994/5/25

N2 - Two components of the sialyl oligosaccharides were separated from milk of the tammar wallaby (Macropus eugenii) by gel filtration and ion exchange chromatography. Their molecular weights, estimated by gel filtration on HPLC using 5 mM triethylamine-acetate buffer (pH 5.0), were approx. 3,000. Their monosaccharide compositions, determined by GC analysis after methanolysis and by colorimetric assay, were (Glc)1(Gal)9(GlcNAc)2(Neu5Ac)1, and (Glc)1(Gal)8(GlcNAc)2(Neu5Ac)2. Their chemical structures were further elucidated by 1H-NMR and methylation analysis. The results suggest that their approximate structures are: {A figure is presented}.

AB - Two components of the sialyl oligosaccharides were separated from milk of the tammar wallaby (Macropus eugenii) by gel filtration and ion exchange chromatography. Their molecular weights, estimated by gel filtration on HPLC using 5 mM triethylamine-acetate buffer (pH 5.0), were approx. 3,000. Their monosaccharide compositions, determined by GC analysis after methanolysis and by colorimetric assay, were (Glc)1(Gal)9(GlcNAc)2(Neu5Ac)1, and (Glc)1(Gal)8(GlcNAc)2(Neu5Ac)2. Their chemical structures were further elucidated by 1H-NMR and methylation analysis. The results suggest that their approximate structures are: {A figure is presented}.

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KW - Milk

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KW - Sialyl Oligosaccharide

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