Chemical characterization of sialyl oligosaccharides isolated from tammar wallaby (Macropus eugenii) milk

Tadasu Urashima, Tadao Saito, Yuhnagi Tsuji, Yasuko Taneda, Toshihide Takasawa, Michael Messer

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Two components of the sialyl oligosaccharides were separated from milk of the tammar wallaby (Macropus eugenii) by gel filtration and ion exchange chromatography. Their molecular weights, estimated by gel filtration on HPLC using 5 mM triethylamine-acetate buffer (pH 5.0), were approx. 3,000. Their monosaccharide compositions, determined by GC analysis after methanolysis and by colorimetric assay, were (Glc)1(Gal)9(GlcNAc)2(Neu5Ac)1, and (Glc)1(Gal)8(GlcNAc)2(Neu5Ac)2. Their chemical structures were further elucidated by 1H-NMR and methylation analysis. The results suggest that their approximate structures are: {A figure is presented}.

Original languageEnglish
Pages (from-to)64-72
Number of pages9
JournalBBA - General Subjects
Volume1200
Issue number1
DOIs
Publication statusPublished - 1994 May 25

Keywords

  • (M. eugenii)
  • Higher Oligosaccharide
  • Marsupial
  • Milk
  • NMR-H
  • Sialyl Oligosaccharide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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