TY - JOUR
T1 - Chemical characterization of sialyl oligosaccharides isolated from tammar wallaby (Macropus eugenii) milk
AU - Urashima, Tadasu
AU - Saito, Tadao
AU - Tsuji, Yuhnagi
AU - Taneda, Yasuko
AU - Takasawa, Toshihide
AU - Messer, Michael
N1 - Funding Information:
We thank Dr. B. Green for supplingt ammarw al-laby milk. This work was supportedb y a grant from AkiyamaL ife ScienceC ommitee.
PY - 1994/5/25
Y1 - 1994/5/25
N2 - Two components of the sialyl oligosaccharides were separated from milk of the tammar wallaby (Macropus eugenii) by gel filtration and ion exchange chromatography. Their molecular weights, estimated by gel filtration on HPLC using 5 mM triethylamine-acetate buffer (pH 5.0), were approx. 3,000. Their monosaccharide compositions, determined by GC analysis after methanolysis and by colorimetric assay, were (Glc)1(Gal)9(GlcNAc)2(Neu5Ac)1, and (Glc)1(Gal)8(GlcNAc)2(Neu5Ac)2. Their chemical structures were further elucidated by 1H-NMR and methylation analysis. The results suggest that their approximate structures are: {A figure is presented}.
AB - Two components of the sialyl oligosaccharides were separated from milk of the tammar wallaby (Macropus eugenii) by gel filtration and ion exchange chromatography. Their molecular weights, estimated by gel filtration on HPLC using 5 mM triethylamine-acetate buffer (pH 5.0), were approx. 3,000. Their monosaccharide compositions, determined by GC analysis after methanolysis and by colorimetric assay, were (Glc)1(Gal)9(GlcNAc)2(Neu5Ac)1, and (Glc)1(Gal)8(GlcNAc)2(Neu5Ac)2. Their chemical structures were further elucidated by 1H-NMR and methylation analysis. The results suggest that their approximate structures are: {A figure is presented}.
KW - (M. eugenii)
KW - Higher Oligosaccharide
KW - Marsupial
KW - Milk
KW - NMR-H
KW - Sialyl Oligosaccharide
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U2 - 10.1016/0304-4165(94)90028-0
DO - 10.1016/0304-4165(94)90028-0
M3 - Article
C2 - 8186234
AN - SCOPUS:0028358195
VL - 1200
SP - 64
EP - 72
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0006-3002
IS - 1
ER -