Chemical characterization of sialyl oligosaccharides in milk of the Japanese black bear, Ursus thibetanus japonicus

Tadasu Urashima, Tadashi Nakamura, Kumi Teramoto, Ikichi Arai, Tadao Saito, Takeshi Komatsu, Toshio Tsubota

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Sialyl oligosaccharides were separated from two samples of Japanese black bear milk by extraction with chloroform/methanol, gel filtration on Bio Gel P-2, ion exchange chromatography on DEAE-Sephadex A-50 and high-performance liquid chromatography (HPLC) on a TSK gel Amido-80 column. They were characterized by 1H-NMR spectroscopy. The structures of four sialyl oligosaccharides separated from the milk were the following:Neu5Ac(α2-3)Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3){Gal(α1-3) Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-6)}Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3){Gal(α1-3) [Fuc(α1-2)]Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-6)}Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3)[Neu5Ac(α2-6) Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc

Original languageEnglish
Pages (from-to)587-595
Number of pages9
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume139
Issue number4
DOIs
Publication statusPublished - 2004 Dec

Keywords

  • B antigen
  • Japanese black bear
  • Japanese black bear milk
  • Milk oligosaccharides
  • Sialyl milk oligosaccharides
  • Ursidae
  • Ursus thibetanus japonicus
  • α-Gal epitope

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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