Abstract
Sialyl oligosaccharides were separated from two samples of Japanese black bear milk by extraction with chloroform/methanol, gel filtration on Bio Gel P-2, ion exchange chromatography on DEAE-Sephadex A-50 and high-performance liquid chromatography (HPLC) on a TSK gel Amido-80 column. They were characterized by 1H-NMR spectroscopy. The structures of four sialyl oligosaccharides separated from the milk were the following:Neu5Ac(α2-3)Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3){Gal(α1-3) Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-6)}Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3){Gal(α1-3) [Fuc(α1-2)]Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-6)}Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3)[Neu5Ac(α2-6) Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc
| Original language | English |
|---|---|
| Pages (from-to) | 587-595 |
| Number of pages | 9 |
| Journal | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| Volume | 139 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2004 Dec |
Keywords
- B antigen
- Japanese black bear
- Japanese black bear milk
- Milk oligosaccharides
- Sialyl milk oligosaccharides
- Ursidae
- Ursus thibetanus japonicus
- α-Gal epitope
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Molecular Biology
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Cite this
Urashima, T., Nakamura, T., Teramoto, K., Arai, I., Saito, T., Komatsu, T., & Tsubota, T. (2004). Chemical characterization of sialyl oligosaccharides in milk of the Japanese black bear, Ursus thibetanus japonicus. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 139(4), 587-595. https://doi.org/10.1016/j.cbpc.2004.07.012