TY - JOUR
T1 - Chemical characterization of sialyl oligosaccharides in milk of the Japanese black bear, Ursus thibetanus japonicus
AU - Urashima, Tadasu
AU - Nakamura, Tadashi
AU - Teramoto, Kumi
AU - Arai, Ikichi
AU - Saito, Tadao
AU - Komatsu, Takeshi
AU - Tsubota, Toshio
N1 - Funding Information:
This study was partially supported by a grant from The 21st Century COE Program (A-1), Ministry of Education, Culture, Sports, Science, and Technology, Japan.
PY - 2004/12
Y1 - 2004/12
N2 - Sialyl oligosaccharides were separated from two samples of Japanese black bear milk by extraction with chloroform/methanol, gel filtration on Bio Gel P-2, ion exchange chromatography on DEAE-Sephadex A-50 and high-performance liquid chromatography (HPLC) on a TSK gel Amido-80 column. They were characterized by 1H-NMR spectroscopy. The structures of four sialyl oligosaccharides separated from the milk were the following:Neu5Ac(α2-3)Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3){Gal(α1-3) Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-6)}Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3){Gal(α1-3) [Fuc(α1-2)]Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-6)}Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3)[Neu5Ac(α2-6) Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc
AB - Sialyl oligosaccharides were separated from two samples of Japanese black bear milk by extraction with chloroform/methanol, gel filtration on Bio Gel P-2, ion exchange chromatography on DEAE-Sephadex A-50 and high-performance liquid chromatography (HPLC) on a TSK gel Amido-80 column. They were characterized by 1H-NMR spectroscopy. The structures of four sialyl oligosaccharides separated from the milk were the following:Neu5Ac(α2-3)Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3){Gal(α1-3) Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-6)}Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3){Gal(α1-3) [Fuc(α1-2)]Gal(β1-4)[Fuc(α1-3)]GlcNAc(β1-6)}Gal(β1-4) GlcNeu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3)[Neu5Ac(α2-6) Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc
KW - B antigen
KW - Japanese black bear
KW - Japanese black bear milk
KW - Milk oligosaccharides
KW - Sialyl milk oligosaccharides
KW - Ursidae
KW - Ursus thibetanus japonicus
KW - α-Gal epitope
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U2 - 10.1016/j.cbpc.2004.07.012
DO - 10.1016/j.cbpc.2004.07.012
M3 - Article
C2 - 15581791
AN - SCOPUS:9744242785
VL - 139
SP - 587
EP - 595
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 4
ER -