Chemical characterization of milk oligosaccharides of the common wombat (Vombatus ursinus)

Kentaro Hirayama, Epi Taufik, Megumi Kikuchi, Tadashi Nakamura, Kenji Fukuda, Tadao Saito, Keith Newgrain, Brian Green, Michael Messer, Tadasu Urashima

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Previous structural characterizations of marsupial milk oligosaccharides have been performed in the tammar wallaby, red kangaroo, koala, common brushtail possum and the eastern quoll. To clarify the homology and heterogeneity of milk oligosaccharides among marsupial species, which could provide information on their evolution, the oligosaccharides of wombat milk carbohydrate were characterized in this study. Neutral and acidic oligosaccharides were isolated from the carbohydrate fractions of two samples of milk of the common wombat and characterized by 1H-nuclear magnetic resonance spectroscopy. The structures of six neutral saccharides were found to be Gal(β1-4)Glc (lactose), Gal(β1-3)Gal(β1-4)Glc (3'-galactosyllactose), Gal(β1-3)Gal(β1-3)Gal(β1-4)Glc (3',3”-digalactosyllactose), Gal(β1-3)Gal(β1-3)Gal(β1-3)Gal(β1-4)Glc, Gal(β1-3)Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (galactosyl lacto-N-novopentaose I) and Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (lacto-N-novooctaose), while those of six acidic saccharides were Neu5Ac(α2-3)Gal(β1-3)Gal(β1-4)Glc. (sialyl 3'-galactosyllactose), Neu5Ac(α2-3)Gal(β1-3)Gal(β1-3)Gal(β1-4)Glc (sialyl 3',3”-digalactosyllactose), Neu5Ac(α2-3)Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (sialyl lacto-N-novopentaose a), Gal(β1-3)[Neu5Ac(α2-3)Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (sialyl lacto-N-novopentaose c), Neu5Ac(α2-3)Gal(β1-3)Gal(β1-3)Gal(β1-3)Gal(β1-4)Glc,, Neu5Ac(α2-3)Gal(β1-3)Gal(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc and Gal(β1-3)Gal(β1-3)[Neu5Ac(α2-3)Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc. In addition, small amounts of sulfated oligosaccharides but no oligosaccharides containing Neu5Gc or α(2–6) linked Neu5Ac were detected.

Original languageEnglish
Pages (from-to)1167-1177
Number of pages11
JournalAnimal Science Journal
Volume87
Issue number9
DOIs
Publication statusPublished - 2016 Sep 1

Keywords

  • Vombatus ursinus
  • common wombat
  • marsupial
  • milk oligosaccharide

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Chemical characterization of milk oligosaccharides of the common wombat (Vombatus ursinus)'. Together they form a unique fingerprint.

  • Cite this

    Hirayama, K., Taufik, E., Kikuchi, M., Nakamura, T., Fukuda, K., Saito, T., Newgrain, K., Green, B., Messer, M., & Urashima, T. (2016). Chemical characterization of milk oligosaccharides of the common wombat (Vombatus ursinus). Animal Science Journal, 87(9), 1167-1177. https://doi.org/10.1111/asj.12566