Chemical characterization of milk oligosaccharides of the brown bear, Ursus arctos yesoensis

Tadasu Urashima, Yohko Kusaka, Tadashi Nakamura, Tadao Saito, Naoko Maeda, Michael Messer

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Two tri-, two hexa- and two deca-saccharides were isolated from bear milk by chloroform/methanol extraction and gel filtration. The oligosaccharides were characterized, mainly by 1H-NMR, as follows: Gal α 1-3Gal β 1-4Glc (α 3'-galactosyllactose); Fuc α 1-2Gal β 1-4Glc (2'-fucosyllactose); Gal α 1-3Gal β 1-4[Fuc α 1-3]GlcNAc β 1-3Gal β 1-4Glc (monofucosylhexasaccharide); Fuc α 1-2Gal β 1-4[Fuc α 1-3]GlcNAc β 1-3Gal β 1-4Glc (difucosylhexasaccharide); Gal α 1-3Gal β 1-4[Fuc α 1-3]GlcNAc β 1 (6) (Gal β 1-4Glc (difucosyldecasaccharide) Gal α 1-3Gal β 1-4[Fuc α 1-3]GlcNAc β 1 (3); Fuc α 1-2Gal β 1-4[Fuc α 1-3]GlcNAc β 1 (6) (Gal β 1-4Glc (tetrafucosyldecasaccharide)) (3) Fuc α 1-2Gal β 1-4[Fuc α 1-3]GlcNAc β 1. Both trisaccharides were present in bear milk at a higher concentration than lactose.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume1334
Issue number2-3
DOIs
Publication statusPublished - 1997 Mar 15

Keywords

  • (Bear)
  • Chemical structure
  • H-;
  • Milk
  • NMR
  • Oligosaccharide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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