Characterization of V3 loop-binding protein(s) of Molt-4 and U937 cells

Y. Xu, T. Murakami, S. Kawase, T. Uchiyama, T. Hattori

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8 Citations (Scopus)

Abstract

The V3 loop in gp120 of human immunodeficiency virus type 1 (HIV-1) is known as a principal neutralizing and cell-tropic determinant. Biotinylated synthetic V3 loop peptides derived from three different HIV-1 strains were used as ligands to identify the cell surface counterreceptor, which may participate in the infection of HIV-1. Two different cell lines, Molt-4 and U937, and three V3 loop peptides derived from LAV(ELI), HTLV-III(MN), and HTLV-IIIB strains were used. The binding of HTLV-IIIB-derived peptide to the cell surface was confirmed using 125I-labeled surface proteins of both cell lines. The relative molecular mass of the major radioactive band on the autoradiogram was 32-33 kDa in both cell lines. A protein was purified from the plasma membrane fraction of Molt-4 cells using affinity columns coupled with three different V3 loop peptides. Two major polypeptides (32 and 33 kDa) were eluted from the affinity column. Size-exclusion chromatography showed that the protein migrated as a single peak with a molecular mass of 130 kDa. These proteins were separated by reversed-phase chromatography, which indicated that the 32-kDa protein is more hydrophobic than the 33-kDa protein in Molt-4 cells. A similar but not identical 130-kDa protein with 32- and 33- kDa polypeptides were also purified from U937 cells. These findings indicate that HIV-1 utilizes a tetrameric protein on the surface of Molt-4 and U937 cells on infection.

Original languageEnglish
Pages (from-to)563-570
Number of pages8
JournalAIDS Research and Human Retroviruses
Volume11
Issue number5
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Immunology
  • Virology

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    Xu, Y., Murakami, T., Kawase, S., Uchiyama, T., & Hattori, T. (1995). Characterization of V3 loop-binding protein(s) of Molt-4 and U937 cells. AIDS Research and Human Retroviruses, 11(5), 563-570.