Characterization of two tobacco cell lines selected to grow in the presence of either ionic Al or insoluble Al-phosphate

Kunihiko Ojima, Hiroyuki Koyama, Rika Suzuki, Tomoyuki Yamaya

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A tobacco cell line tolerant to ionic A1 (AIT) was selected at a frequency of 10-6to 10-7by plating the wild-type cells on agar medium containing 2.5 mm AICI3and 0.1 mM phosphate at pH 4.0. Another cell line which is able to grow with insoluble Al-phosphate as a sole source of phosphate (IPU) was also selected by subculturing the wild-type cells for more than 10 passages in suspension culture in the presence of 2 mM phosphate and 4 mM A1C13at pH 5.0. The IPU cell line also tolerated ionic A1 but the AIT line showed poor growth with insoluble Al-phosphate as a sole source of phosphate. Both cell lines excreted citrate into the media. The rate of citrate excretion by the IPU cell line, on a fresh weight basis, was higher than that by the AIT and wild-type cell lines.

Original languageEnglish
Pages (from-to)545-551
Number of pages7
JournalSoil Science and Plant Nutrition
Volume35
Issue number4
DOIs
Publication statusPublished - 1989 Dec

Keywords

  • A1 tolerance
  • Insoluble A1 phosphate
  • Ionic A1
  • Tobacco cells

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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