Abstract
A tobacco cell line tolerant to ionic A1 (AIT) was selected at a frequency of 10-6to 10-7by plating the wild-type cells on agar medium containing 2.5 mm AICI3and 0.1 mM phosphate at pH 4.0. Another cell line which is able to grow with insoluble Al-phosphate as a sole source of phosphate (IPU) was also selected by subculturing the wild-type cells for more than 10 passages in suspension culture in the presence of 2 mM phosphate and 4 mM A1C13at pH 5.0. The IPU cell line also tolerated ionic A1 but the AIT line showed poor growth with insoluble Al-phosphate as a sole source of phosphate. Both cell lines excreted citrate into the media. The rate of citrate excretion by the IPU cell line, on a fresh weight basis, was higher than that by the AIT and wild-type cell lines.
Original language | English |
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Pages (from-to) | 545-551 |
Number of pages | 7 |
Journal | Soil Science and Plant Nutrition |
Volume | 35 |
Issue number | 4 |
DOIs | |
Publication status | Published - 1989 Dec |
Externally published | Yes |
Keywords
- A1 tolerance
- Insoluble A1 phosphate
- Ionic A1
- Tobacco cells
ASJC Scopus subject areas
- Soil Science
- Plant Science