Characterization of the Streptococcus mutans pyruvate formate-lyase (PFL)-activating enzyme gene by complementary reconstitution of the in vitro PFL-reactivating system

Yasuhito Yamamoto, Yutaka Sato, Shoko Takahashi-Abbe, Nobuhiro Takahashi, Harutoshi Kizaki

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The act gene was identified and an act mutant as well as the pfl mutant was constructed in Streptococcus mutans. Pyruvate formate-lyase (PFL) activity was regenerated with the mixture of the respective cell extracts from these mutants by complementary reconstitution of the in vitro reactivating system. The S. mutans act gene encoded the sole enzyme able to activate the PFL protein in this organism.

Original languageEnglish
Pages (from-to)4773-4777
Number of pages5
JournalInfection and immunity
Volume68
Issue number8
DOIs
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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