Characterization of the S-RNase promoters from sweet cherry (Prunus avium L.)

Takako Ishizaka, Hideaki Nakano, Takashi Suzuki, Hiroyasu Kitashiba

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Genomic DNA fragments containing the S3-, S4, and S6-RNase genes were isolated from the sweet cherry (Prunus avium L.) and sequenced. Comparison of the 5′-flanking sequences of these three S-RNases indicated that a highly conserved region (designated CR) existed just upstream from the putative TATA boxes. We postulate that CR contains cis-regulatory element(s) involved in pistil expression. To examine the activity of the isolated S-RNase promoters of sweet cherry in the pistil, we transiently introduced approximately 650-bp fragments of the S4- and S6-RNase promoters fused to β-glucuronidase (GUS) gene into the pistil of the petunia using a particle bombardment technique. Histochemical analysis showed that the 5′-flanking region of each S-RNase was active in the pistil. This suggests that cis-regulatory element(s) for pistil-specific expression may exist(s) within the 650-bp region upstream from the TATA box in the sweet cherry S-RNase promoter.

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalGenes and Genetic Systems
Volume78
Issue number2
DOIs
Publication statusPublished - 2003 Apr

Keywords

  • 5′-flanking region
  • Prunus avium L.
  • S-RNase
  • Self-incompatibility
  • Sweet cherry

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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