Characterization of the promoter of the mouse preproorexin gene

Haruno Kato; Hiroshi Hosoda; Toru Fukuda; Shoichi Masushige; Satoshi Kida

doi:10.1271/bbb.60100

Characterization of the promoter of the mouse preproorexin gene

Haruno Kato, Hiroshi Hosoda, Toru Fukuda, Shoichi Masushige, Satoshi Kida

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The neuropeptide Orexin is involved in the regulation of the sleep-awake cycle and feeding behavior. We isolated a 22-kb genomic clone containing the 5′ flanking region of the mouse Orexin promoter. We determined that the transcription start site (+1) is located 96 nucleotides upstream of the initiation codon. The start site region contained consensus sequences corresponding to the transcription initiator and TATA box. Analysis of promoter activity using PC12 cells showed that regions between -13 and +112 and between -1,868 and -780 contained nerve growth factor (NGF)-responsive positive regulatory element and a negative regulatory element respectively.

Original language	English
Pages (from-to)	840-843
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	71
Issue number	3
DOIs	https://doi.org/10.1271/bbb.60100
Publication status	Published - 2007
Externally published	Yes

Keywords

Nerve growth factor (NGF)
Orexin
PC12 cell
Promoter
Transcription

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.60100

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title = "Characterization of the promoter of the mouse preproorexin gene",

abstract = "The neuropeptide Orexin is involved in the regulation of the sleep-awake cycle and feeding behavior. We isolated a 22-kb genomic clone containing the 5′ flanking region of the mouse Orexin promoter. We determined that the transcription start site (+1) is located 96 nucleotides upstream of the initiation codon. The start site region contained consensus sequences corresponding to the transcription initiator and TATA box. Analysis of promoter activity using PC12 cells showed that regions between -13 and +112 and between -1,868 and -780 contained nerve growth factor (NGF)-responsive positive regulatory element and a negative regulatory element respectively.",

keywords = "Nerve growth factor (NGF), Orexin, PC12 cell, Promoter, Transcription",

author = "Haruno Kato and Hiroshi Hosoda and Toru Fukuda and Shoichi Masushige and Satoshi Kida",

note = "Funding Information: This work was supported by a Grant-in Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, a Grant-in Aid for High Technology Research from the Ministry of Education, Culture, Sports, Science and Technology, Priority Areas–Molecular Brain Science– from the Ministry of Education, Culture, Sports, Science and Technology and the Research Grant for Nervous and Mental Disorders from the Ministry of Health, Labor and Welfare, and by the Urakami Foundation.",

year = "2007",

doi = "10.1271/bbb.60100",

language = "English",

volume = "71",

pages = "840--843",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "3",

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T1 - Characterization of the promoter of the mouse preproorexin gene

AU - Kato, Haruno

AU - Hosoda, Hiroshi

AU - Fukuda, Toru

AU - Masushige, Shoichi

AU - Kida, Satoshi

N1 - Funding Information: This work was supported by a Grant-in Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, a Grant-in Aid for High Technology Research from the Ministry of Education, Culture, Sports, Science and Technology, Priority Areas–Molecular Brain Science– from the Ministry of Education, Culture, Sports, Science and Technology and the Research Grant for Nervous and Mental Disorders from the Ministry of Health, Labor and Welfare, and by the Urakami Foundation.

PY - 2007

Y1 - 2007

N2 - The neuropeptide Orexin is involved in the regulation of the sleep-awake cycle and feeding behavior. We isolated a 22-kb genomic clone containing the 5′ flanking region of the mouse Orexin promoter. We determined that the transcription start site (+1) is located 96 nucleotides upstream of the initiation codon. The start site region contained consensus sequences corresponding to the transcription initiator and TATA box. Analysis of promoter activity using PC12 cells showed that regions between -13 and +112 and between -1,868 and -780 contained nerve growth factor (NGF)-responsive positive regulatory element and a negative regulatory element respectively.

AB - The neuropeptide Orexin is involved in the regulation of the sleep-awake cycle and feeding behavior. We isolated a 22-kb genomic clone containing the 5′ flanking region of the mouse Orexin promoter. We determined that the transcription start site (+1) is located 96 nucleotides upstream of the initiation codon. The start site region contained consensus sequences corresponding to the transcription initiator and TATA box. Analysis of promoter activity using PC12 cells showed that regions between -13 and +112 and between -1,868 and -780 contained nerve growth factor (NGF)-responsive positive regulatory element and a negative regulatory element respectively.

KW - Nerve growth factor (NGF)

KW - Orexin

KW - PC12 cell

KW - Promoter

KW - Transcription

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DO - 10.1271/bbb.60100

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VL - 71

SP - 840

EP - 843

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 3

ER -

Characterization of the promoter of the mouse preproorexin gene

Abstract

Keywords

ASJC Scopus subject areas

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