Characterization of the promoter of the mouse preproorexin gene

Haruno Kato; Hiroshi Hosoda; Toru Fukuda; Shoichi Masushige; Satoshi Kida

doi:10.1271/bbb.60100

Characterization of the promoter of the mouse preproorexin gene

Haruno Kato, Hiroshi Hosoda, Toru Fukuda, Shoichi Masushige, Satoshi Kida

University Hospital

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The neuropeptide Orexin is involved in the regulation of the sleep-awake cycle and feeding behavior. We isolated a 22-kb genomic clone containing the 5′ flanking region of the mouse Orexin promoter. We determined that the transcription start site (+1) is located 96 nucleotides upstream of the initiation codon. The start site region contained consensus sequences corresponding to the transcription initiator and TATA box. Analysis of promoter activity using PC12 cells showed that regions between -13 and +112 and between -1,868 and -780 contained nerve growth factor (NGF)-responsive positive regulatory element and a negative regulatory element respectively.

Original language	English
Pages (from-to)	840-843
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	71
Issue number	3
DOIs	https://doi.org/10.1271/bbb.60100
Publication status	Published - 2007 Mar 30

Keywords

Nerve growth factor (NGF)
Orexin
PC12 cell
Promoter
Transcription

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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abstract = "The neuropeptide Orexin is involved in the regulation of the sleep-awake cycle and feeding behavior. We isolated a 22-kb genomic clone containing the 5′ flanking region of the mouse Orexin promoter. We determined that the transcription start site (+1) is located 96 nucleotides upstream of the initiation codon. The start site region contained consensus sequences corresponding to the transcription initiator and TATA box. Analysis of promoter activity using PC12 cells showed that regions between -13 and +112 and between -1,868 and -780 contained nerve growth factor (NGF)-responsive positive regulatory element and a negative regulatory element respectively.",

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T1 - Characterization of the promoter of the mouse preproorexin gene

AU - Kato, Haruno

AU - Hosoda, Hiroshi

AU - Fukuda, Toru

AU - Masushige, Shoichi

AU - Kida, Satoshi

PY - 2007/3/30

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N2 - The neuropeptide Orexin is involved in the regulation of the sleep-awake cycle and feeding behavior. We isolated a 22-kb genomic clone containing the 5′ flanking region of the mouse Orexin promoter. We determined that the transcription start site (+1) is located 96 nucleotides upstream of the initiation codon. The start site region contained consensus sequences corresponding to the transcription initiator and TATA box. Analysis of promoter activity using PC12 cells showed that regions between -13 and +112 and between -1,868 and -780 contained nerve growth factor (NGF)-responsive positive regulatory element and a negative regulatory element respectively.

AB - The neuropeptide Orexin is involved in the regulation of the sleep-awake cycle and feeding behavior. We isolated a 22-kb genomic clone containing the 5′ flanking region of the mouse Orexin promoter. We determined that the transcription start site (+1) is located 96 nucleotides upstream of the initiation codon. The start site region contained consensus sequences corresponding to the transcription initiator and TATA box. Analysis of promoter activity using PC12 cells showed that regions between -13 and +112 and between -1,868 and -780 contained nerve growth factor (NGF)-responsive positive regulatory element and a negative regulatory element respectively.

KW - Nerve growth factor (NGF)

KW - Orexin

KW - PC12 cell

KW - Promoter

KW - Transcription

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