Characterization of the promoter of the mouse preproorexin gene

Haruno Kato, Hiroshi Hosoda, Toru Fukuda, Shoichi Masushige, Satoshi Kida

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


The neuropeptide Orexin is involved in the regulation of the sleep-awake cycle and feeding behavior. We isolated a 22-kb genomic clone containing the 5′ flanking region of the mouse Orexin promoter. We determined that the transcription start site (+1) is located 96 nucleotides upstream of the initiation codon. The start site region contained consensus sequences corresponding to the transcription initiator and TATA box. Analysis of promoter activity using PC12 cells showed that regions between -13 and +112 and between -1,868 and -780 contained nerve growth factor (NGF)-responsive positive regulatory element and a negative regulatory element respectively.

Original languageEnglish
Pages (from-to)840-843
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Issue number3
Publication statusPublished - 2007
Externally publishedYes


  • Nerve growth factor (NGF)
  • Orexin
  • PC12 cell
  • Promoter
  • Transcription

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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