Characterization of the physicochemical properties of phospholipid vesicles prepared in CO2/water systems at high pressure

Hidemi Nakamura; Shogo Taguchi; Keishi Suga; Keita Hayashi; Ho Sup Jung; Hiroshi Umakoshi

doi:10.1116/1.4928722

Characterization of the physicochemical properties of phospholipid vesicles prepared in CO₂/water systems at high pressure

Hidemi Nakamura, Shogo Taguchi, Keishi Suga, Keita Hayashi, Ho Sup Jung, Hiroshi Umakoshi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Phospholipid vesicles were prepared by the nonsolvent method using high-pressure CO₂/water systems. The membrane properties of vesicles prepared at different pressures and temperatures were mainly characterized based on analysis of the membrane fluidity and membrane polarity, using the fluorescent probes 1,6-diphenyl-1,3,5-hexatriene and 6-dodecanoyl-N,N-dimethyl-2-naphthylamine, respectively. The CO_2(liquid)/water_(liquid) and the CO_{2(supercritical)}/water_(liquid) two-phase (heterogeneous) systems resulted in the formation of vesicles with high yield (ca. 85%-88%). The membrane fluidity and polarity of the vesicles were similar to those of liposomes prepared by the conventional method. It is suggested that high-pressure CO2 can be used to form an appropriate hydrophobic-hydrophilic interface where phospholipid molecules as a self-assembled membrane.

Original language	English
Article number	031005
Pages (from-to)	1-6
Number of pages	6
Journal	Biointerphases
Volume	10
Issue number	3
DOIs	https://doi.org/10.1116/1.4928722
Publication status	Published - 2015
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Biomaterials
Materials Science(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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abstract = "Phospholipid vesicles were prepared by the nonsolvent method using high-pressure CO2/water systems. The membrane properties of vesicles prepared at different pressures and temperatures were mainly characterized based on analysis of the membrane fluidity and membrane polarity, using the fluorescent probes 1,6-diphenyl-1,3,5-hexatriene and 6-dodecanoyl-N,N-dimethyl-2-naphthylamine, respectively. The CO2(liquid)/water(liquid) and the CO2(supercritical)/water(liquid) two-phase (heterogeneous) systems resulted in the formation of vesicles with high yield (ca. 85%-88%). The membrane fluidity and polarity of the vesicles were similar to those of liposomes prepared by the conventional method. It is suggested that high-pressure CO2 can be used to form an appropriate hydrophobic-hydrophilic interface where phospholipid molecules as a self-assembled membrane.",

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AU - Nakamura, Hidemi

AU - Taguchi, Shogo

AU - Suga, Keishi

AU - Hayashi, Keita

AU - Jung, Ho Sup

AU - Umakoshi, Hiroshi

PY - 2015

Y1 - 2015

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Characterization of the physicochemical properties of phospholipid vesicles prepared in CO₂/water systems at high pressure

Abstract

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