Characterization of the physicochemical properties of phospholipid vesicles prepared in CO2/water systems at high pressure

Hidemi Nakamura, Shogo Taguchi, Keishi Suga, Keita Hayashi, Ho Sup Jung, Hiroshi Umakoshi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Phospholipid vesicles were prepared by the nonsolvent method using high-pressure CO2/water systems. The membrane properties of vesicles prepared at different pressures and temperatures were mainly characterized based on analysis of the membrane fluidity and membrane polarity, using the fluorescent probes 1,6-diphenyl-1,3,5-hexatriene and 6-dodecanoyl-N,N-dimethyl-2-naphthylamine, respectively. The CO2(liquid)/water(liquid) and the CO2(supercritical)/water(liquid) two-phase (heterogeneous) systems resulted in the formation of vesicles with high yield (ca. 85%-88%). The membrane fluidity and polarity of the vesicles were similar to those of liposomes prepared by the conventional method. It is suggested that high-pressure CO2 can be used to form an appropriate hydrophobic-hydrophilic interface where phospholipid molecules as a self-assembled membrane.

Original languageEnglish
Article number031005
Pages (from-to)1-6
Number of pages6
Issue number3
Publication statusPublished - 2015
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomaterials
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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