Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a

Chie Kaneko, Shigetsugu Hatakeyama, Masaki Matsumoto, Masayoshi Yada, Keiko Nakayama, Keiichi I. Nakayama

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

UFD2a is a mammalian homolog of Saccharomyces cerevisiae Ufd2, originally described as an E4 ubiquitination factor. UFD2a belongs to the U-box family of ubiquitin ligases (E3s) and likely functions as both an E3 and E4. We have isolated and characterized the mouse gene (Ube4b) for UFD2a. A full-length (∼5700bp) Ube4b cDNA was isolated and the corresponding gene spans 100kb, comprising 27 exons. Luciferase reporter gene analysis of the 5 flanking region of Ube4b revealed that nucleotides -1018 to -943 (relative to the translation initiation site) possess promoter activity. This functional sequence contains two putative Sp1 binding sites but not a TATA box. Immunoblot and immunohistochemical analyses revealed that UFD2a is expressed predominantly in the neuronal tissues. We also show that UFD2a interacts with VCP (a AAA-family ATPase) that is thought to mediate protein folding. These data implicate UFD2a in the degradation of neuronal proteins by the ubiquitin-proteasome pathway.

Original languageEnglish
Pages (from-to)297-304
Number of pages8
JournalBiochemical and biophysical research communications
Volume300
Issue number2
DOIs
Publication statusPublished - 2003 Jan 10
Externally publishedYes

Keywords

  • Proteolysis
  • U-box protein
  • UFD2a
  • Ubiquitin
  • Ubiquitin ligase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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