Characterization of the molecular defects in RAB27A, caused by RAB27A missense mutations found in patients with Griscelli syndrome

Philippe Bahadoran, Roser Busca, Christine Chiaverini, Wendy Westbroek, Jo Lambert, Karine Bille, Gaëlle Valony, Mitsunori Fukuda, Jean Marie Naeyaert, Jean Paul Ortonne, Robert Ballotti

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Rab27a plays a pivotal role in the transport of melanosomes to dendrite tips of melanocytes and mutations in RAB27A, which impair melanosome transport cause the pigmentary dilution and the immune deficiency found in several patients with Griscelli syndrome (GS). Interestingly, three GS patients present single homozygous missense mutations in RAB27A, leading to W73G, L130P, and A152P transitions that affect highly conserved residues among Rab proteins. However, the functional consequences of these mutations have not been studied. In the present report, we evaluated the effect of overexpression of these mutants on melanosome, melanophilin, and myosin-Va localization in B16 melanoma cells. Then we studied several key parameters for Rab27a function, including GTP binding and interaction with melanophilin/myosin-Va complex, which links melanosomes to the actin network. Our results showed that Rab27a-L130P cannot bind GTP, does not interact with melanophilin, and consequently cannot allow melanosome transport on the actin filaments. Interestingly, Rab27a-W73G binds GTP but does not interact with melanophilin. Thus, Rab27a-W73G cannot support the actin-dependent melanosome transport. Finally, Rab27a-A152P binds both GTP and melanophilin. However, Rab27a-A152P does not allow melanosome transport and acts as a dominant negative mutant, because its overexpression, in B16 melanoma cells, mimics a GS phenotype. Hence, the interaction of Rab27a with melanophilin/myosin-Va is not sufficient to ensure a correct melanosome transport. Our results pointed to an unexpected complexity of Rab27a function and open the way to the search for new Rab27a effectors or regulators that control the transport of Rab27a-dependent vesicles.

Original languageEnglish
Pages (from-to)11386-11392
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number13
DOIs
Publication statusPublished - 2003 Mar 28

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Characterization of the molecular defects in RAB27A, caused by RAB27A missense mutations found in patients with Griscelli syndrome'. Together they form a unique fingerprint.

Cite this