Characterization of the Lectin from the Skin Mucus of the Kingklip Genypterus capensis

Michitoshi Toda, Rina Goto-Nance, Koji Muramoto, Hisao Kamiya

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    A mitogenic lectin in the skin mucus of the kingklip Genypterus capensis was purified by a combination of affinity adsorption on the glutaraldehyde-fixed rabbit erythrocyte ghosts and chromatofocusing on PBE 94 gels. The lectin was a glycoprotein having a molecular weight of 28-34 kDa which was composed with two homogenous subunit (13.7 kDa). The isoelectric point was 6.45. Kingklip lectin agglutinated rabbit and horse eryttirocytes but not human ABO erythrocytes. The hemagglutinating activity was calcium ion-dependent and was inhibited effectively by asialo-mucin Type I and also by simple sugars such as N-acetyl-D-glucosamine. The amino-terminal sequence of the kingklip lectin was identified as Ser-Met-Cys-Asn-Cys-Gly-Trp-Ser-Gln-Phe-Ala-His-Leu-Ala-Tyr-Leu-Leu-Arg-Ser-Lys- Ala-.

    Original languageEnglish
    Pages (from-to)138-141
    Number of pages4
    JournalFisheries Science
    Volume62
    Issue number1
    DOIs
    Publication statusPublished - 1996 Feb

    Keywords

    • Genypterus capensis
    • Kingklip
    • Lectin
    • Mitogenic
    • Skin mucus

    ASJC Scopus subject areas

    • Aquatic Science

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