Characterization of the Anti-Bovine Podoplanin Monoclonal Antibody PMab-44

Shinji Yamada, Ryusuke Honma, Mika K. Kaneko, Takuro Nakamura, Miyuki Yanaka, Noriko Saidoh, Michiaki Takagi, Satoru Konnai, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A type I transmembrane sialoglycoprotein podoplanin (PDPN) is expressed in several normal cells, including podocytes of the kidney, type I alveolar cells of the lung, and lymphatic endothelial cells. We recently produced an anti-bovine PDPN (bovPDPN) monoclonal antibody (mAb), PMab-44, by immunizing mice with recombinant proteins of bovPDPN. In this study, we determined the critical epitope of PMab-44 for the recognition of bovPDPN using many deletion mutants and point mutants of bovPDPN. Flow cytometric analyses revealed that the epitope of PMab-44 was Glu46-Thr50, which corresponds to platelet aggregation-stimulating (PLAG) domain-3. The important amino acids in the PMab-44 epitope were determined to be Glu46, Tyr48, and Thr50. Western blot analysis also confirmed these results, indicating that the PLAG domain of bovPDPN is also important in immunogenicity for producing useful anti-PDPN mAbs.

Original languageEnglish
Pages (from-to)129-134
Number of pages6
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume36
Issue number3
DOIs
Publication statusPublished - 2017 Jun

Keywords

  • Bovine PDPN
  • PMab-44
  • Podoplanin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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