Characterization of the 5′-regulatory region of rat Reg I gene

Hikari Miyashita; Hideto Yonekura; Michiaki Unno; Yoshihisa Suzuki; Takuo Watanabe; Shigeki Moriizumi; Shin Takasawa; Hiroshi Okamoto

doi:10.1016/0167-4781(94)90281-X

Characterization of the 5′-regulatory region of rat Reg I gene

Hikari Miyashita, Hideto Yonekura, Michiaki Unno, Yoshihisa Suzuki, Takuo Watanabe, Shigeki Moriizumi, Shin Takasawa, Hiroshi Okamoto

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We report here the characterization of the 5′-regulatory region of rat Reg I gene encoding a growth stimulating factor for pancreatic β-cells. Transient expression assays of the 5′-flanking region/luciferase fusion gene in AR4-2J cells showed that the -304 -237 region contained positive cis-acting elements. Gel shift assays using AR4-2J and rat pancreas nuclear extracts showed the formation of a specific complex with the -256 -237 oligonucleotide.

Original language	English
Pages (from-to)	241-243
Number of pages	3
Journal	BBA - Gene Structure and Expression
Volume	1219
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(94)90281-X
Publication status	Published - 1994 Sep 13

Keywords

(Rat)
CANNTG motif
Gel shift assay
Luciferase assay
Reg gene

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(94)90281-X

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title = "Characterization of the 5′-regulatory region of rat Reg I gene",

abstract = "We report here the characterization of the 5′-regulatory region of rat Reg I gene encoding a growth stimulating factor for pancreatic β-cells. Transient expression assays of the 5′-flanking region/luciferase fusion gene in AR4-2J cells showed that the -304 -237 region contained positive cis-acting elements. Gel shift assays using AR4-2J and rat pancreas nuclear extracts showed the formation of a specific complex with the -256 -237 oligonucleotide.",

keywords = "(Rat), CANNTG motif, Gel shift assay, Luciferase assay, Reg gene",

author = "Hikari Miyashita and Hideto Yonekura and Michiaki Unno and Yoshihisa Suzuki and Takuo Watanabe and Shigeki Moriizumi and Shin Takasawa and Hiroshi Okamoto",

note = "Funding Information: This work was supported in part by grants-in-aid from the Ministry of Education, Science and Culture, Japan and the Takeda Medical Research Foundation, and was submitted in partial fulfillment of the degree of Doctor of Medical Science at Tohoku University (H. Funding Information: Miyashita). H. Miyashita and M. Unno are recipients of a fellowship from the Japan Society for the Promotion of Science.",

year = "1994",

month = sep,

day = "13",

doi = "10.1016/0167-4781(94)90281-X",

language = "English",

volume = "1219",

pages = "241--243",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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publisher = "Elsevier BV",

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T1 - Characterization of the 5′-regulatory region of rat Reg I gene

AU - Miyashita, Hikari

AU - Yonekura, Hideto

AU - Unno, Michiaki

AU - Suzuki, Yoshihisa

AU - Watanabe, Takuo

AU - Moriizumi, Shigeki

AU - Takasawa, Shin

AU - Okamoto, Hiroshi

N1 - Funding Information: This work was supported in part by grants-in-aid from the Ministry of Education, Science and Culture, Japan and the Takeda Medical Research Foundation, and was submitted in partial fulfillment of the degree of Doctor of Medical Science at Tohoku University (H. Funding Information: Miyashita). H. Miyashita and M. Unno are recipients of a fellowship from the Japan Society for the Promotion of Science.

PY - 1994/9/13

Y1 - 1994/9/13

N2 - We report here the characterization of the 5′-regulatory region of rat Reg I gene encoding a growth stimulating factor for pancreatic β-cells. Transient expression assays of the 5′-flanking region/luciferase fusion gene in AR4-2J cells showed that the -304 -237 region contained positive cis-acting elements. Gel shift assays using AR4-2J and rat pancreas nuclear extracts showed the formation of a specific complex with the -256 -237 oligonucleotide.

AB - We report here the characterization of the 5′-regulatory region of rat Reg I gene encoding a growth stimulating factor for pancreatic β-cells. Transient expression assays of the 5′-flanking region/luciferase fusion gene in AR4-2J cells showed that the -304 -237 region contained positive cis-acting elements. Gel shift assays using AR4-2J and rat pancreas nuclear extracts showed the formation of a specific complex with the -256 -237 oligonucleotide.

KW - (Rat)

KW - CANNTG motif

KW - Gel shift assay

KW - Luciferase assay

KW - Reg gene

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DO - 10.1016/0167-4781(94)90281-X

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AN - SCOPUS:0027981465

VL - 1219

SP - 241

EP - 243

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 1

ER -

Characterization of the 5′-regulatory region of rat Reg I gene

Abstract

Keywords

ASJC Scopus subject areas

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