Characterization of the 5′-regulatory region of rat Reg I gene

Hikari Miyashita; Hideto Yonekura; Michiaki Unno; Yoshihisa Suzuki; Takuo Watanabe; Shigeki Moriizumi; Shin Takasawa; Hiroshi Okamoto

doi:10.1016/0167-4781(94)90281-X

Characterization of the 5′-regulatory region of rat Reg I gene

Hikari Miyashita, Hideto Yonekura, Michiaki Unno, Yoshihisa Suzuki, Takuo Watanabe, Shigeki Moriizumi, Shin Takasawa, Hiroshi Okamoto

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We report here the characterization of the 5′-regulatory region of rat Reg I gene encoding a growth stimulating factor for pancreatic β-cells. Transient expression assays of the 5′-flanking region/luciferase fusion gene in AR4-2J cells showed that the -304 -237 region contained positive cis-acting elements. Gel shift assays using AR4-2J and rat pancreas nuclear extracts showed the formation of a specific complex with the -256 -237 oligonucleotide.

Original language	English
Pages (from-to)	241-243
Number of pages	3
Journal	BBA - Gene Structure and Expression
Volume	1219
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(94)90281-X
Publication status	Published - 1994 Sep 13

Keywords

(Rat)
CANNTG motif
Gel shift assay
Luciferase assay
Reg gene

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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title = "Characterization of the 5′-regulatory region of rat Reg I gene",

abstract = "We report here the characterization of the 5′-regulatory region of rat Reg I gene encoding a growth stimulating factor for pancreatic β-cells. Transient expression assays of the 5′-flanking region/luciferase fusion gene in AR4-2J cells showed that the -304 -237 region contained positive cis-acting elements. Gel shift assays using AR4-2J and rat pancreas nuclear extracts showed the formation of a specific complex with the -256 -237 oligonucleotide.",

keywords = "(Rat), CANNTG motif, Gel shift assay, Luciferase assay, Reg gene",

author = "Hikari Miyashita and Hideto Yonekura and Michiaki Unno and Yoshihisa Suzuki and Takuo Watanabe and Shigeki Moriizumi and Shin Takasawa and Hiroshi Okamoto",

year = "1994",

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doi = "10.1016/0167-4781(94)90281-X",

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T1 - Characterization of the 5′-regulatory region of rat Reg I gene

AU - Miyashita, Hikari

AU - Yonekura, Hideto

AU - Unno, Michiaki

AU - Suzuki, Yoshihisa

AU - Watanabe, Takuo

AU - Moriizumi, Shigeki

AU - Takasawa, Shin

AU - Okamoto, Hiroshi

PY - 1994/9/13

Y1 - 1994/9/13

N2 - We report here the characterization of the 5′-regulatory region of rat Reg I gene encoding a growth stimulating factor for pancreatic β-cells. Transient expression assays of the 5′-flanking region/luciferase fusion gene in AR4-2J cells showed that the -304 -237 region contained positive cis-acting elements. Gel shift assays using AR4-2J and rat pancreas nuclear extracts showed the formation of a specific complex with the -256 -237 oligonucleotide.

AB - We report here the characterization of the 5′-regulatory region of rat Reg I gene encoding a growth stimulating factor for pancreatic β-cells. Transient expression assays of the 5′-flanking region/luciferase fusion gene in AR4-2J cells showed that the -304 -237 region contained positive cis-acting elements. Gel shift assays using AR4-2J and rat pancreas nuclear extracts showed the formation of a specific complex with the -256 -237 oligonucleotide.

KW - (Rat)

KW - CANNTG motif

KW - Gel shift assay

KW - Luciferase assay

KW - Reg gene

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