Characterization of the 5′-regulatory region of rat Reg I gene

Hikari Miyashita, Hideto Yonekura, Michiaki Unno, Yoshihisa Suzuki, Takuo Watanabe, Shigeki Moriizumi, Shin Takasawa, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We report here the characterization of the 5′-regulatory region of rat Reg I gene encoding a growth stimulating factor for pancreatic β-cells. Transient expression assays of the 5′-flanking region/luciferase fusion gene in AR4-2J cells showed that the -304 -237 region contained positive cis-acting elements. Gel shift assays using AR4-2J and rat pancreas nuclear extracts showed the formation of a specific complex with the -256 -237 oligonucleotide.

Original languageEnglish
Pages (from-to)241-243
Number of pages3
JournalBBA - Gene Structure and Expression
Volume1219
Issue number1
DOIs
Publication statusPublished - 1994 Sep 13

Keywords

  • (Rat)
  • CANNTG motif
  • Gel shift assay
  • Luciferase assay
  • Reg gene

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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