Characterization of T Cell Receptors of Th1 Cells Infiltrating Inflamed Skin of a Novel Murine Model of Palladium-Induced Metal Allergy

Hiroshi Kobayashi, Kenichi Kumagai, Takanori Eguchi, Hiroaki Shigematsu, Kazutaka Kitaura, Mitsuko Kawano, Tatsuya Horikawa, Satsuki Suzuki, Takaji Matsutani, Kouetsu Ogasawara, Yoshiki Hamada, Ryuji Suzuki

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17 Citations (Scopus)

Abstract

Metal allergy is categorized as a delayed-type hypersensitivity reaction, and is characterized by the recruitment of lymphocytes into sites of allergic inflammation. Because of the unavailability of suitable animal models for metal allergy, the role of T cells in the pathogenesis of metal allergy has not been explored. Thus, we developed a novel mouse model for metal allergy associated with infiltration of T cells by multiple injections of palladium (Pd) plus lipopolysaccharide into the footpad. Using this model, we characterized footpad-infiltrating T cells in terms of phenotypic markers, T cell receptor (TCR) repertoires and cytokine expression. CD3+ CD4+ T cells accumulated in the allergic footpads 7 days after Pd challenge. The expression levels of CD25, interleukin-2, interferon-γ and tumor necrosis factor, but not interleukin-4 and interleukin-5, increased in the footpads after challenge, suggesting CD4+ T helper 1 (Th1) cells locally expanded in response to Pd. Infiltrated T cells in the footpads frequently expressed AV18-1 and BV8-2 T cell receptor (TCR) chains compared with T cells in the lymph nodes and exhibited oligoclonality. T-cell clones identified from Pd-allergic mouse footpads shared identical CDR3 sequences containing AV18-1 and BV8-2. These results suggest that TCR AV18-1 and BV8-2 play dominant and critical parts in the antigen specificity of Pd-specific Th1 cells.

Original languageEnglish
Article numbere76385
JournalPloS one
Volume8
Issue number10
DOIs
Publication statusPublished - 2013 Oct 3

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Kobayashi, H., Kumagai, K., Eguchi, T., Shigematsu, H., Kitaura, K., Kawano, M., Horikawa, T., Suzuki, S., Matsutani, T., Ogasawara, K., Hamada, Y., & Suzuki, R. (2013). Characterization of T Cell Receptors of Th1 Cells Infiltrating Inflamed Skin of a Novel Murine Model of Palladium-Induced Metal Allergy. PloS one, 8(10), [e76385]. https://doi.org/10.1371/journal.pone.0076385