Characterization of symptom determinants in two mutants of cucumber mosaic virus Y strain, causing distinct mild green mosaic symptoms in tobacco

M. Sugiyama, H. Sato, A. Karasawa, S. Hase, H. Takahashi, Y. Ehara

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Two mutants of Cucumber mosaic virus, CMV(Y/GM1) and CMV(Y/GM2), which induced mild green mosaic symptoms in tobacco, were isolated from plants regenerated from tobacco leaves with yellow mosaic symptoms originally infected with the yellow strain of CMV [CMV(Y)]. Although the appearance of mild green mosaic symptoms in tobacco infected with CMV(Y/GM2) was unstable, CMV(Y/GM3) derived from CMV(Y/GM2) reproducibly induced mild green mosaic symptoms in tobacco similar to CMV(Y/GM1). A comparison of the deduced amino acid sequences of the coat proteins of CMV(Y), CMV(Y/GM1) and CMV(Y/GM3), showed single amino acid substitutions from Thr to Ile at position 124 in the CMV(Y/GM1) coat protein and from Val to Ile at position 111 in the CMV(Y/GM3) coat protein. When the amino acid at the 124 or 111 position in the CMV(Y) coat protein was changed to Ile at the cDNA level, CMV RNA3 transcribed in vitro from each cDNA induced mild green mosaic symptoms in tobacco after inoculation with in vitro transcribed CMV(Y) RNA1 and RNA2. The results indicated that amino acids at positions 111 and 124 in the coat protein were responsible for the phenotypic changes caused by the two CMV isolates. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)85-90
Number of pages6
JournalPhysiological and Molecular Plant Pathology
Volume56
Issue number2
DOIs
Publication statusPublished - 2000 Jan 1

Keywords

  • Coat protein
  • Cucumber mosaic virus
  • Mutant
  • Symptom expression

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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