Characterization of steroidogenic enzyme expression in aldosterone-producing adenoma: A comparison with various human adrenal tumors

Ikki Sakuma, Sachiko Suematsu, Yoko Matsuzawa, Jun Saito, Masao Omura, Takashi Maekawa, Yasuhiro Nakamura, Hironobu Sasano, Tetsuo Nishikawa

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

We analyzed the expression profiles of several steroidogenic enzymes in normal adrenals, aldosterone-producing adenomas (APA), cortisol-producing adenomas combined with Cushing's syndrome (CPA) or with subclinical Cushing's syndrome (SCPA), and nonfunctioning adrenal adenomas (NFA) to clarify the nature and characteristics of steroidogenesis in APA. Clinical data were collected for all subjects. In resected adrenal glands (normal adrenals, APA, CPA, SCPA, and NFA), the mRNA expression levels of the CYP17, HSD3B2, CYP11B1, and CYP11B2 genes were studied using real-time quantitative PCR and immunohistochemistry. The CYP11B2 mRNA level in APA was significantly higher than that in other groups. The CYP17/HSD3B2 ratio for mRNA in APA was significantly lower than those in the other groups. Low ratio of CYP17/HSD3B2 with high expression of CYP11B2 seems to explain steroidogenic characteristics of APA.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
Journalendocrine journal
Volume60
Issue number3
DOIs
Publication statusPublished - 2013

Keywords

  • Aldosterone
  • Aldosterone-producing adenoma
  • Immunohistochemistry
  • Steroidogenic enzymes
  • mRNA expression

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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