Characterization of start-up performance and archaeal community shifts during anaerobic self-degradation of waste-activated sludge

Takuro Kobayashi, Daisuke Yasuda, Yu You Li, Kengo Kubota, Hideki Harada, Han Qing Yu

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

Successful start-up strategy for anaerobic digestion of waste-activated sludge using internal inoculum and relationship between the shift of methanogenic community and the digester performance during start-up was investigated. Combination of TS control of inoculum and batch operation during early days enabled the successful start-up operation without serious volatile fatty acid accumulation, followed by the stable continuous operation. However, the propionate degradation was rate-limiting step during the batch operation. The results of real-time quantitative polymerase chain reaction analysis suggested that there was a correlation between the population of the genus Methanosarcina and the methane production rate coupled with acetate consumption during batch operation, and the results of terminal-restriction fragment length polymorphism (T-RFLP) revealed that the increasing intensity of T-RF peaks of hydrogenotrophic methanogens was associated with a decrease in the level of C3-acids.

Original languageEnglish
Pages (from-to)4981-4988
Number of pages8
JournalBioresource Technology
Volume100
Issue number21
DOIs
Publication statusPublished - 2009 Nov 1

Keywords

  • Anaerobic digestion
  • Archaeal community shift
  • Inoculum
  • Start-up

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Fingerprint Dive into the research topics of 'Characterization of start-up performance and archaeal community shifts during anaerobic self-degradation of waste-activated sludge'. Together they form a unique fingerprint.

Cite this