Abstract
Selenium (Se) is well known to be essential for cell culture when using a serum-free medium, but not when a medium containing serum is used. This finding suggests that serum contains some usable form of Se. To identify the Se-supplier, T-lymphoma (Jurkat) cells were cultured for 3 days in the presence of human serum immunodepleted of Se-containing serum protein, selenoprotein P or extracellular glutathione peroxidase. The Se-dependent enzyme activities (glutathione peroxidases and thioredoxin reductase) and Se content within the cells markedly decreased only when cultured with selenoprotein P-depleted serum. Compared with other Se-containing proteins, the addition of purified selenoprotein P to the selenoprotein P-depleted serum or a serum-free medium was the most effective for the recovery of cellular glutathione peroxidase activity (index of Se status). These results suggest that selenoprotein P functions as a Se-supply protein, delivering Se to the cells.
Original language | English |
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Pages (from-to) | 5746-5751 |
Number of pages | 6 |
Journal | European Journal of Biochemistry |
Volume | 269 |
Issue number | 22 |
DOIs | |
Publication status | Published - 2002 |
Externally published | Yes |
Keywords
- Glutathione peroxidase
- Selenium
- Selenoprotein
ASJC Scopus subject areas
- Biochemistry