Characterization of S tester lines in Brassica oleracea: Polymorphism of restriction fragment length of SLG homologues and isoelectric points of S-locus glycoproteins

K. Okazaki, M. Kusaba, D. J. Ockendon, T. Nishio

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Forty three S tester lines of Brassica oleracea were characterized using DNA and protein gel-blotting analyses. DNA gel-blot analysis of HindIII-digested genomic DNA with class-I and class-II SLG probes revealed that 40 lines could be classified as class-I S haplotypes while three lines could be classified as class-II S haplotypes. The band patterns in the S tester lines were highly polymorphic. Although the S tester lines typically showed two bands corresponding to SLG and SRK in the analysis with the class-I SLG probe, only one band was observed in the S24 homozygote. This band was identified as SRK, suggesting that this haplotype has no class-I SLG band. In the analysis using the class-II SLG probe, one plant yielded a different band pattern from the known class-II haplotypes, S2, S5 and S15. Unexpectedly, this plant was reciprocally cross-incompatible with the S2 haplotype. Therefore, it was designated as S(2-b). We found an S13 haplotype having a restriction fragment length polymorphism different from that of the S13 homozygotes of the S tester line. These findings indicate that S homozygous lines with the same S specificity do not necessarily show the same band pattern in the DNA gel-blot analysis. Soluble stigma proteins of 32 S homozygotes were separated by isoelectric focusing and detected using anti-S22SLG antiserum. S haplotype-specific bands were detected in 27 S homozygotes but not in five S homozygotes, including the S24 homozygote. This is consistent with the observation that the S24 haplotype had no SLG band.

Original languageEnglish
Pages (from-to)1329-1334
Number of pages6
JournalTheoretical and Applied Genetics
Volume98
Issue number8
DOIs
Publication statusPublished - 1999

Keywords

  • Brassica oleracea
  • IEF
  • RFLP
  • S haplotype
  • Self-incompatibility

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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