Characterization of phosphate uptake in primary cultures of rat hepatocytes

H. Arai, W. W. Wells

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Phosphate uptake across the plasma membrane of rat hepatocytes was characterized in primary cultures of rat hepatocytes. Phosphate uptake by cultured rat hepatocytes consisted of a Na+-dependent and Na+-independent component. A major portion of the uptake was Na+-dependent, which was saturable and exhibited Michaelis-Menten kinetics. Kinetic analysis showed that the apparent Km value for phosphate at 137 mM Na+ was 0.25 mM and Vmax was 13.3 pmol/μg protein for 30 min. Hill coefficient was 1.76. This study is the first to demonstrate the presence of a phosphate carrier on the plasma membrane of hepatocytes.

Original languageEnglish
Pages (from-to)563-571
Number of pages9
JournalBiochemistry International
Volume20
Issue number3
Publication statusPublished - 1990 Apr 13
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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