Characterization of peptides in sea anemone venom collected by a novel procedure

Elena L.A. Malpezzi, JoséCarlos de Freitas, Koji Muramoto, Hisao Kamiya

    Research output: Contribution to journalArticlepeer-review

    47 Citations (Scopus)

    Abstract

    Peptide neurotoxins were isolated from the venom obtained by electrical stimulation of the sea anemone Bunodosoma caissarum. This technique allows almost pure venom to be collected, and the animals to survive. Three neurotoxins (assayed on crustacean nerves) were isolated by gel filtration and reversed-phase high performance liquid chromatography. Hemolysins were also detected in the venom. The amino acid sequence of a major neurotoxin BcIII was determined. BcIII has 48 amino acid residues with six half-cystine residues. This sequence has homology with the type 1 long sea anemone neurotoxins. Two minor toxins (BcI and II) have similar amino acid composition and amino-terminal sequences to BcIII.

    Original languageEnglish
    Pages (from-to)853-864
    Number of pages12
    JournalToxicon
    Volume31
    Issue number7
    DOIs
    Publication statusPublished - 1993 Jul

    ASJC Scopus subject areas

    • Toxicology

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